Mouse Chemerin Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Chemerin in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 372402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Chemerin
    Thr17-Ser156
    Accession # Q9DD06
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Chemerin (Catalog # 2325-CM) under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize Chemerin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human Chem R23. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Mouse Chemerin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody. Recombinant Mouse Chemerin (Catalog # 2325-CM) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human Chem R23 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Chemerin (100 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Chemerin Monoclonal Antibody (Catalog # MAB2325). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chemerin

Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1‑3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (aa) (2, 3). Chemerin is synthesized as a 162 aa precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4‑6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C-terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17‑156) (5‑7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148‑156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84% and 82% aa sequence identity to human, rat and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).

  • References:
    1. Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
    2. Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
    3. Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
    4. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
    5. Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
    6. Busmann, A. et al. (2004) J. Chromatog. B 811:217.
    7. Meder, W. et al. (2003) FEBS Lett. 555:495.
    8. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.
  • Long Name:
    Retinoic Acid Receptor Responder Protein 2
  • Entrez Gene IDs:
    5919 (Human); 71660 (Mouse)
  • Alternate Names:
    Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. The role of chemerin and ChemR23 in stimulating the invasion of squamous oesophageal cancer cells
    Authors: JD Kumar, S Kandola, L Tiszlavicz, Z Reisz, GJ Dockray, A Varro
    Br. J. Cancer, 2016;114(10):1152-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Increased expression of chemerin in squamous esophageal cancer myofibroblasts and role in recruitment of mesenchymal stromal cells.
    Authors: Kumar, J Dinesh, Holmberg, Chris, Kandola, Sandhir, Steele, Islay, Hegyi, Peter, Tiszlavicz, Laszlo, Jenkins, Rosalind, Beynon, Robert J, Peeney, David, Giger, Olivier, Alqahtani, Ahlam, Wang, Timothy, Charvat, Trevor T, Penfold, Mark, Dockray, Graham J, Varro, Andrea
    PLoS ONE, 2014;9(7):e104877.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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