Mouse Chordin Antibody Summary
Accession # Q9Z0E2
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB758).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Chordin DuoSet ELISA Kit (Catalog # DY758) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse Chordin ELISA Standard Curve. Recombinant Mouse Chordin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB758) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse Chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for Chordin in organogenesis and homeostasis.
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