Mouse CLEC9a Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB67762-100
MAB67762-SP
Mouse CLEC9a Antibody in Flow Cytometry
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Mouse CLEC9a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CLEC9a in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2456A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CLEC9a
Lys57-Ile264
Accession # EDK99924
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rabbit Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67762) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Anti-Mouse Integrin alpha X/CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69051R). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC9a

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.

Long Name
C-type Lectin-like Receptor 9
Entrez Gene IDs
283420 (Human); 232414 (Mouse)
Alternate Names
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

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