Mouse CLEC9a Antibody Summary
Accession # EDK99924
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rabbit Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67762) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Anti-Mouse Integrin alpha X/CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69051R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse CLEC9a Antibody
There are currently no reviews for this product. Be the first to review Mouse CLEC9a Antibody and earn rewards!
Have you used Mouse CLEC9a Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image