Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Neutralization, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 7H11
View all CLEC9a Primary Antibodies »

Product Specifications

Immunogen

RBL‑2H3 rat basophilic leukemia cell line expressing mouse CLEC9a fused to an HA epitope
Accession # Q8BRU4

Specificity

Detects mouse CLEC9a.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CLEC9a Antibody

Detection of CLEC9a antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CLEC9a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse CLEC9a Monoclonal Antibody (Catalog # MAB67761) or (B) Rat IgG1Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse CLEC9a Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µL/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

Sancho D., et al. (2008) J. Clin. Invest. 118:2098.

Western Blot

Sancho D., et al. (2008) J. Clin. Invest. 118:2098.

Reviewed Applications

Read 1 review rated 5 using MAB67761 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC9a

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.

Long Name

C-type Lectin-like Receptor 9

Alternate Names

CD370, DNGR1

Entrez Gene IDs

283420 (Human); 232414 (Mouse)

Gene Symbol

CLEC9A

UniProt

Q8BRU4 (Mouse)

Additional CLEC9a Products

Product Documents for Mouse CLEC9a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CLEC9a Antibody

For research use only

Citations for Mouse CLEC9a Antibody

Customer Reviews for Mouse CLEC9a Antibody (1)

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  • Mouse CLEC9a Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Endothelial cells
    Species: Mouse
    Verified Customer | Posted 04/13/2022
    Mouse CLEC9a Antibody MAB67761

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail