Mouse Collagen XIII  alpha 1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Collagen XIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) COL13A1v4 is observed, and less than 1% cross-reactivity with rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Collagen XIII alpha 1
    Glu107-Gln565
    Accession # AAH34164
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Collagen XIII  alpha 1 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collagen XIII  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XIII  alpha 1 at approximately 85‑95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Collagen XIII  alpha 1 in Mouse Embryo. Collagen XIII  alpha 1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Collagen XIII  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen XIII alpha 1

Collagen XIII alpha 1 is an 85-95 kDa protein in the type 2 transmembrane collagen family (1). Mature mouse Collagen XIII alpha 1 consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment, and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non‑collagenous regions (2, 3). Within comparable regions of the ECD, mouse Collagen XIII alpha 1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII alpha 1, respectively. Mouse Collagen XIII alpha 1 is extensively spliced, with some isoforms showing a tissue specific distribution (2, 4). Collagen XIII alpha 1 is widely expressed during development and in the adult (4, 5). It localizes to intercellular adherens junctions and cell-matrix focal adhesions (6, 7). Collagen XIII  alpha 1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation (2, 8). Trimerization involves triple helix formation within the collagenous domains, although portions of the non‑collagenous regions can also form coiled coils (8‑10). The ECD of trimeric Collagen XIII alpha 1 is an extended rod-like structure with two flexible hinges that correspond to non‑collagenous regions (11). Collagen XIII alpha 1 clusters in cholesterol-rich domains on the plasma membrane (2, 12), and it can be cleaved from the cell surface or intracellularly by a furin-like protease (12). Collagen XIII alpha 1 binds the extracellular matrix molecules fibronectin, heparin, integrin  alpha 1, nidogen‑2, and perlecan (11, 13). The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation (14).

 

  • References:
    1. Franzke, C.-W. et al. (2005) J. Biol. Chem. 280:4005.
    2. Hagg, P. et al. (1998) J. Biol. Chem. 273:15590.
    3. Pihlajaniemi, T. and M. Tamminen, 1990, J. Biol. Chem. 265:16922.
    4. Peltonen, S. et al. (1997) DNA Cell Biol. 16:227.
    5. Sund, M. et al. (2001) Matrix Biol. 20:215.
    6. Peltonen, S. et al. (1999) J. Invest. Dermatol. 113:635.
    7. Hagg, P. et al. (2001) Matrix Biol. 19:727.
    8. Snellman, A. et al. (2000) J. Biol. Chem. 275:8936.
    9. Latvanlehto, A. et al. (2003) J. Biol. Chem. 278:37590.
    10. Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
    11. Tu, H. et al. (2002) J. Biol. Chem. 277:23092.
    12. Vaisanen, T. et al. (2006) J. Biol. Chem. 281:33352.
    13. Nykvist, P. et al. (2000) J. Biol. Chem. 275:8255.
    14. Vaisanen, M.-R. et al. (2006) Biochem. J. 393:43.
  • Entrez Gene IDs:
    1305 (Human); 12817 (Mouse); 499431 (Rat)
  • Alternate Names:
    COL13A1; collagen alpha-1(XIII) chain; Collagen XIII alpha 1; collagen, type XIII, alpha 1; FLJ42485
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