Mouse CX3CL1/Fractalkine Quantikine ELISA Kit

(6 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    0.32 ng/mL
  • Assay Range
    0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse Fractalkine (chemokine and extracellular domains)
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Fractalkine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fractalkine in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Fractalkine chemokine domain and antibodies raised against the recombinant protein. Results obtained for naturally occurring soluble mouse Fractalkine containing the chemokine domain and the mucin stalk domain showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fractalkine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.190.421.70.170.310.92


The recovery of mouse Fractalkine spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 101 94-106
EDTA Plasma (n=6) 95 83-109
Heparin Plasma (n=6) 95 81-110
Serum (n=6) 98 91-107
Tissue Homogenates (n=1) 100 100
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Fractalkine in each matrix were diluted with Calibrator Diluent and assayed.
Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CX3CL1/Fractalkine
CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
    • Entrez Gene IDs
      6376 (Human); 20312 (Mouse); 89808 (Rat);
    • Alternate Names
      ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Fibroblast growth factor receptor 1 activation in mammary tumor cells promotes macrophage recruitment in a CX3CL1-dependent manner.
      Authors: Reed, Johanna, Stone, Matthew, Beadnell, Thomas C, Ryu, Yungil, Griffin, Timothy, Schwertfeger, Kathryn
      PLoS ONE, 2012;7(9):e45877.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Ozone inhalation promotes CX3CR1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation.
      Authors: Tighe RM, Li Z, Potts EN, Frush S, Liu N, Gunn MD, Foster WM, Noble PW, Hollingsworth JW
      J. Immunol., 2011;187(9):4800-8.
      Species: Mouse
      Sample Type: BALF
    3. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    4. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
      Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
      J. Immunol., 2008;181(6):4208-18.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    5. The arthritis severity quantitative trait loci Cia4 and Cia6 regulate neutrophil migration into inflammatory sites and levels of TNF-alpha and nitric oxide.
      Authors: Laragione T, Yarlett NC, Brenner M, Mello A, Sherry B, Miller EJ, Metz CN, Gulko PS
      J. Immunol., 2007;178(4):2344-51.
      Species: Rat
      Sample Type: Air Pouch Exudate
    6. Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
      Authors: Ren T, Chen Q, Tian Z, Wei H
      Biochem. Biophys. Res. Commun., 2007;364(4):978-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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