CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
R&D Systems | Catalog # MCX310
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.32 ng/mL
Assay Range
0.62-40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
The Quantikine Mouse Fractalkine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fractalkine in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Fractalkine chemokine domain and antibodies raised against the recombinant protein. Results obtained for naturally occurring soluble mouse Fractalkine containing the chemokine domain and the mucin stalk domain showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fractalkine.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse Fractalkine (chemokine and extracellular domains)
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 41 | 41 | 41 |
| Mean (pg/mL) | 1.9 | 5.2 | 18.6 | 1.4 | 5.5 | 20.0 |
| Standard Deviation | 0.19 | 0.42 | 1.7 | 0.17 | 0.31 | 0.92 |
| CV% | 10.0 | 8.1 | 9.1 | 12.1 | 5.6 | 4.6 |
Recovery for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
The recovery of mouse Fractalkine spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 101 | 94-106 |
| EDTA Plasma (n=6) | 95 | 83-109 |
| Heparin Plasma (n=6) | 95 | 81-110 |
| Serum (n=6) | 98 | 91-107 |
| Tissue Homogenates (n=1) | 100 | 100 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Fractalkine in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
Mouse CX3CL1/Fractalkine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CX3CL1/Fractalkine
Alternate Names
FKN, Fractalkine, Neurotactin
Gene Symbol
CX3CL1
Additional CX3CL1/Fractalkine Products
Product Documents for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse CX3CL1/Fractalkine Quantikine ELISA Kit (MCX310):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Chemokine Superfamily Pathway: Human/Mouse Ligand-Receptor Interactions
