Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL10/IP‑10/CRG‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IP‑10, recombinant mouse (rm) PF4, rmMIG, rmLIX, and rmI-TAC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CXCL10/IP‑10/CRG‑2 (R&D Systems, Catalog # 466-CR)
    Ile22-Pro98
    Accession # Q548V9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB466)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF466)
  • ELISA Standard
     
    Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog # 466-CR)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CXCL10/IP‑10/CRG‑2 in Mouse Thymus. CXCL10/IP‑10/CRG‑2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse
CXCL10/IP‑10/CRG‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF466) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
    3. Sgadari, C. et al. (1996) Blood, 87:3877.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA detection
  2. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
    Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
    Nat Immunol, 2015;16(8):850-8.
  3. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
    Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
    J Immunol, 2015;196(3):1227-38.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA detection
  4. Colonic patch and colonic SILT development are independent and differentially regulated events.
    Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
    Mucosal Immunol, 2013;6(3):511-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  5. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
    Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
    Mol Ther, 2012;20(12):2315-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
    Authors: Meng Y, Efimova EV, Hamzeh KW
    Mol. Ther., 2012;20(5):1046-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
    Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
    Arthritis Rheum., 2006;54(4):1174-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005;107(5):2112-22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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