Mouse CXCL10/IP-10/CRG-2 Antibody

(14 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
    Ile22-Pro98
    Accession # P17515
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse CXCL10 by Western Blot.
Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CXCL10 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Chemotaxis Induced by CXCL10/CRG‑2 and Neutral­ization by Mouse CXCL10/
CRG‑2 Antibody.
Recombinant Mouse CXCL10/
CRG‑2 (Catalog # 466‑CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/
CRG‑2 (0.5 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5-25 µg/mL.

Immunohistochemistry

CXCL10/IP‑10/CRG‑2 in Mouse Thymus. CXCL10/IP‑10/CRG‑2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse
CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2
The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
    3. Sgadari, C. et al. (1996) Blood 87:3877.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease
    Authors: X Li, QY Yao, HC Liu, QW Jin, BL Xu, SC Zhang, CT Tu
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017;7(0):46514.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
    J Neuroinflammation, 2016;13(1):298.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  5. Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
    Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
    J Neuroinflammation, 2014;11(0):105.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency.
    Authors: Zeng X, Nagavalli A, Smith C, Howard J, Su M
    J Immunol, 2013;190(8):3895-904.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  7. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
    Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
    Mol Ther, 2012;20(12):2315-25.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
    Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
    Circulation, 2009;119(3):426-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  10. TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
    Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
    J. Immunol., 2007;179(11):7949-58.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
    Authors: Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD
    J. Immunol., 2007;178(12):8090-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Functional Assay
  13. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2007;81(2):783-90.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003;71(5):2634-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 14 Citations
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