Mouse CXCL11/I-TAC Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL11/I‑TAC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CXC1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, 13, 14, recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, 13, 14, 15, recombinant rat CXCL1, 2, CXCL3/CINC2 alpha, CXCL3/CINC-2 beta, 5, or recombinant porcine CXCL8 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 131327
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL11/I-TAC
    Phe22-Met100
    Accession # Q9JHH5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC)
  • Neutralization
    Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 50 ng/mL Recombinant Mouse CXCL11/I‑TAC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL11/I‑TAC and Neutral­ization by Mouse CXCL11/
I‑TAC Antibody.
Recombinant Mouse CXCL11/
I‑TAC (Catalog # 572-MC) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/
I‑TAC (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB572). The ND50 is typically 0.4‑2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL11/I-TAC

CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) precursor protein with a putative 21 aa signal peptide that is cleaved to yield a 79 aa mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

  • References:
    1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
    2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
    3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.
  • Entrez Gene IDs:
    6373 (Human); 56066 (Mouse)
  • Alternate Names:
    beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Rho GTPases control ciliary epithelium cells proliferation and progenitor profile induction in vivo.
    Authors: Del Debbio C, Santos M, Yan C, Ahmad I, Hamassaki D
    Invest Ophthalmol Vis Sci, 2014;55(4):2631-41.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  2. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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