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Mouse Decorin Antibody

Catalog #: AF1060
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1060
AF1060-SP
Detection of Mouse Decorin by Western Blot.
2 Images
Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Decorin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (13 d.p.c., dorsal root ganglion)

Mouse Decorin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Decorin antibody by Western Blot. View Larger

Detection of Mouse Decorin by Western Blot. Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Decorin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Decorin by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

References
  1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Alternate Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

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Citations for Mouse Decorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Abeta amyloidosis
    Authors: R Jiang, U Smailovic, H Haytural, BM Tijms, H Li, RM Haret, G Shevchenko, G Chen, A Abelein, J Gobom, S Frykman, M Sekiguchi, R Fujioka, N Watamura, H Sasaguri, S Nyström, P Hammarströ, TC Saido, V Jelic, S Syvänen, H Zetterberg, B Winblad, J Bergquist, PJ Visser, P Nilsson
    Acta neuropathologica communications, 2022;10(1):96.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Simple gene signature to assess murine fibroblast polarization
    Authors: E Ledoult, M Jendoubi, A Collet, T Guerrier, A Largy, S Speca, S Vivier, F Bray, M Figeac, E Hachulla, M Labalette, F Leprêtre, S Sebda, S Sanges, C Rolando, V Sobanski, S Dubucquoi, D Launay
    Scientific Reports, 2022;12(1):11748.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Tissue-specific smooth muscle cell subtypes identified by transcriptional profiling
    Authors: CJ Lin, RP Mecham
    The international journal of biochemistry & cell biology, 2021;0(0):106055.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019;21(1):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
    Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
    Mol Ther Oncolytics, 2017;6(0):1-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture
    Authors: Y Shen, V Russo, MR Zeglinski, SL Sellers, Z Wu, C Oram, S Santacruz, Y Merkulova, C Turner, K Tauh, H Zhao, T Bozin, L Bohunek, H Zeng, MA Seidman, RC Bleackley, BM McManus, E Ruoslahti, TAH Järvinen, DJ Granville
    Sci Rep, 2017;7(1):15857.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2015;22(2):351-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development.
    Authors: Dupuis L, Kern C
    Dev Dyn, 2014;243(4):601-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells.
    Authors: Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG
    Cell Stem Cell, 2011;9(1):74-86.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
    Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
    Am. J. Pathol., 2011;179(3):1179-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Enhanced macromolecule diffusion deep in tumors after enzymatic digestion of extracellular matrix collagen and its associated proteoglycan decorin.
    Authors: Magzoub M, Jin S, Verkman AS
    FASEB J., 2007;22(1):276-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Isotype Controls

Normal Goat IgG Control

AB-108-C
352 Citations
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Donkey Anti-Goat IgG Antibody

AF109
7 Citations
WB

Donkey Anti-Goat IgG Biotinylated Antibody

BAF109
3 Citations
WB

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
8 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

NL001
27 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
7 Citations
Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
12 Citations
1 Image
Flow

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
3 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
10 Citations
1 Image
Flow

Goat IgG HRP-conjugated Antibody

HAF109
47 Citations
2 Images
WB,Simple Western

Goat IgG HRP-conjugated Antibody

HAF017
53 Citations
1 Image
WB

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
17 Citations
2 Images
WB,IHC

Goat IgG VisUCyte HRP Polymer

VC004
5 Citations
1 Image
IHC

Rabbit Anti-Goat IgG (H+L) Antibody, X Absorbed

R-401-C-ABS
WB,ELISA

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
2 Citations
WB

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