hh Mouse Decorin Antibody AF1060: R&D Systems

Mouse Decorin Antibody

(5 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Decorin
    Gly17-Lys354
    Accession # P28654
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryo (13 d.p.c., dorsal root ganglion)
    • Mouse Decorin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Decorin Antibody (Catalog # AF1060)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Decorin by Western Blot. Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Decorin by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

  • References:
    1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
    2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
  • Entrez Gene IDs:
    1634 (Human); 13179 (Mouse)
  • Alternate Names:
    Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2015;22(2):351-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  2. Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development.
    Authors: Dupuis L, Kern C
    Dev Dyn, 2014;243(4):601-11.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells.
    Authors: Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG
    Cell Stem Cell, 2011;9(1):74-86.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
    Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
    Am. J. Pathol., 2011;179(3):1179-87.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Enhanced macromolecule diffusion deep in tumors after enzymatic digestion of extracellular matrix collagen and its associated proteoglycan decorin.
    Authors: Magzoub M, Jin S, Verkman AS
    FASEB J., 2007;22(1):276-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Acidic

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Worked well on methacarn fixed tissue, Immunohistochemistry.

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Worked well on methacarn fixed tissue
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Images Ratings Applications Species Reviewed By Date Details
Worked well on methacarn fixed tissue Mouse Decorin Antibody AF1060
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Very Good
 Worked well on methacarn fixed tissue Mouse Anonymous 05/18/2017
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Worked well on methacarn fixed tissue Mouse Decorin Antibody AF1060
Worked well on methacarn fixed tissue: Mouse Decorin Antibody [AF1060]

Summary

ApplicationWorked well on methacarn fixed tissue
Sample TestedColon cancer tissue,Colon tissue
SpeciesMouse
Immunohistochemistry Mouse Decorin Antibody AF1060
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Excellent
 IHC Mouse Anonymous 05/19/2016
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Immunohistochemistry Mouse Decorin Antibody AF1060
Immunohistochemistry: Mouse Decorin Antibody [AF1060]

Summary

ApplicationImmunohistochemistry
Sample TestedProstate tissue
SpeciesMouse

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