Detection of Mouse Decorin by Western Blot. Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody |
(Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Decorin by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the |
12-230 kDa separation system.
Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).
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Immunohistochemistry: Mouse Decorin Antibody [AF1060]
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