Detection of Mouse Decorin by Western Blot.
Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Decorin by Simple WesternTM.
Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).
Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
Entrez Gene IDs:
1634 (Human); 13179 (Mouse)
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B
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Read what people are saying who have used Mouse Decorin Antibody.
We have 4 reviews tested in 2 species: Mouse, Rat,Mouse.
We have 4 reviews tested in 4 applications: Immunocytochemistry/Immunofluorescence, Western Blot, Worked well on methacarn fixed tissue, Immunohistochemistry.