Mouse Decorin Antibody

Catalog # Availability Size / Price Qty
AF1060
AF1060-SP
Detection of Mouse Decorin by Western Blot.
2 Images
Product Details
Citations (23)
FAQs
Supplemental Products
Reviews (4)

Mouse Decorin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (13 d.p.c., dorsal root ganglion)

Mouse Decorin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Decorin Biotinylated Antibody (Catalog # BAF1060)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Decorin antibody by Western Blot. View Larger

Detection of Mouse Decorin by Western Blot. Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Decorin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Decorin by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

References
  1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Alternate Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

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Citations for Mouse Decorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. The female syndecan-4−/− heart has smaller cardiomyocytes, augmented insulin/pSer473-Akt/pSer9-GSK-3 beta signaling, and lowered SCOP, pThr308-Akt/Akt and GLUT4 levels
    Authors: Thea Parsberg Støle, Marianne Lunde, Xin Shen, Marita Martinsen, Per Kristian Lunde, Jia Li et al.
    Frontiers in Cell and Developmental Biology
  2. Syndecan-4–/– Mice Have Smaller Muscle Fibers, Increased Akt/mTOR/S6K1 and Notch/HES-1 Pathways, and Alterations in Extracellular Matrix Components
    Authors: Sissel Beate Rønning, Cathrine Rein Carlson, Jan Magnus Aronsen, Addolorata Pisconti, Vibeke Høst, Marianne Lunde et al.
    Frontiers in Cell and Developmental Biology
  3. Accumulation of Extracellular Matrix in Advanced Lesions of Canine Distemper Demyelinating Encephalitis
    Authors: Frauke Seehusen, Seham A. Al-Azreg, Barbara B. Raddatz, Verena Haist, Christina Puff, Ingo Spitzbarth et al.
    PLOS ONE
  4. Astrocyte depletion alters extracellular matrix composition in the demyelinating phase of Theiler’s murine encephalomyelitis
    Authors: Lisa Allnoch, Eva Leitzen, Isabel Zdora, Wolfgang Baumgärtner, Florian Hansmann
    PLOS ONE
  5. Morphological and Extracellular Matrix Changes following Vocal Fold Injury in Mice
    Authors: Masaru Yamashita, Diane M. Bless, Nathan V. Welham
    Cells Tissues Organs (Print)
  6. Decorin interferes with platelet-derived growth factor receptor signaling in experimental hepatocarcinogenesis
    Authors: Kornélia Baghy, Zsolt Horváth, Eszter Regős, Katalin Kiss, Zsuzsa Schaff, Renato V. Iozzo et al.
    FEBS Journal
  7. Circulating cytokine levels in mice with heart failure are etiology dependent
    Authors: Maria Vistnes, Anne Waehre, Ståle Nygård, Ivar Sjaastad, Kristin B. Andersson, Cathrine Husberg et al.
    Journal of Applied Physiology
  8. Lack of Chemokine Signaling through CXCR5 Causes Increased Mortality, Ventricular Dilatation and Deranged Matrix during Cardiac Pressure Overload
    Authors: Anne Waehre, Bente Halvorsen, Arne Yndestad, Cathrine Husberg, Ivar Sjaastad, Ståle Nygård et al.
    PLoS ONE
  9. Xylosyltransferase I mediates the synthesis of proteoglycans with long glycosaminoglycan chains and controls chondrocyte hypertrophy and collagen fibers organization of in the growth plate
    Authors: Taieb, M;Ghannoum, D;Barré, L;Ouzzine, M;
    Cell death & disease
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  10. Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations
    Authors: Y Itoh, V Sahni, SJ Shnider, H McKee, JD Macklis
    Cell Reports, 2023-03-17;42(3):112182.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Increased Proteoglycanases in Pulmonary Valves after Birth Correlate with Extracellular Matrix Maturation and Valve Sculpting
    Authors: LE Dupuis, SE Evins, MC Abell, ME Blakley, SL Horkey, JL Barth, CB Kern
    Journal of cardiovascular development and disease, 2023-01-11;10(1):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Simple gene signature to assess murine fibroblast polarization
    Authors: E Ledoult, M Jendoubi, A Collet, T Guerrier, A Largy, S Speca, S Vivier, F Bray, M Figeac, E Hachulla, M Labalette, F Leprêtre, S Sebda, S Sanges, C Rolando, V Sobanski, S Dubucquoi, D Launay
    Scientific Reports, 2022-07-11;12(1):11748.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Abeta amyloidosis
    Authors: R Jiang, U Smailovic, H Haytural, BM Tijms, H Li, RM Haret, G Shevchenko, G Chen, A Abelein, J Gobom, S Frykman, M Sekiguchi, R Fujioka, N Watamura, H Sasaguri, S Nyström, P Hammarströ, TC Saido, V Jelic, S Syvänen, H Zetterberg, B Winblad, J Bergquist, PJ Visser, P Nilsson
    Acta neuropathologica communications, 2022-07-04;10(1):96.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Tissue-specific smooth muscle cell subtypes identified by transcriptional profiling
    Authors: CJ Lin, RP Mecham
    The international journal of biochemistry & cell biology, 2021-08-16;0(0):106055.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019-02-27;21(1):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture
    Authors: Y Shen, V Russo, MR Zeglinski, SL Sellers, Z Wu, C Oram, S Santacruz, Y Merkulova, C Turner, K Tauh, H Zhao, T Bozin, L Bohunek, H Zeng, MA Seidman, RC Bleackley, BM McManus, E Ruoslahti, TAH Järvinen, DJ Granville
    Sci Rep, 2017-11-20;7(1):15857.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
    Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
    Mol Ther Oncolytics, 2017-05-17;6(0):1-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development.
    Authors: Daquinag, A C, Tseng, C, Salameh, A, Zhang, Y, Amaya-Manzanares, F, Dadbin, A, Florez, F, Xu, Y, Tong, Q, Kolonin, M G
    Cell Death Differ, 2014-10-24;22(2):351-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development.
    Authors: Dupuis L, Kern C
    Dev Dyn, 2014-01-04;243(4):601-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
    Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
    Am. J. Pathol., 2011-06-30;179(3):1179-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells.
    Authors: Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG
    Cell Stem Cell, 2011-06-16;9(1):74-86.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  22. Enhanced macromolecule diffusion deep in tumors after enzymatic digestion of extracellular matrix collagen and its associated proteoglycan decorin.
    Authors: Magzoub M, Jin S, Verkman AS
    FASEB J., 2007-08-29;22(1):276-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Reprogramming alveolar macrophage responses to TGF-beta reveals CCR2+ monocyte activity that promotes bronchiolitis obliterans syndrome
    Authors: Zhiyi Liu, Fuyi Liao, Jihong Zhu, Dequan Zhou, Gyu Seong Heo, Hannah P. Leuhmann et al.
    Journal of Clinical Investigation

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Reviews for Mouse Decorin Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Mouse Decorin Antibody
By Kimberly Alonge on 04/10/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse and Rat

4C overnight, very strong and specific


Mouse Decorin Antibody
By Jim Hsiao on 03/20/2018
Application: WB Sample Tested: Liver tissue Species: Mouse

Primary antibody was diluted in 5% milk/TBST at 1:1000. Blot was incubated in cold room overnight.


Mouse Decorin Antibody
By Anonymous on 05/18/2017
Application: IHC Sample Tested: Colon cancer tissue,Colon tissue Species: Mouse

Mouse Decorin Antibody
By Anonymous on 05/19/2016
Application: IHC Sample Tested: Prostate tissue Species: Mouse