Home / Endoglin/CD105 / Mouse Endoglin/CD105 PE-conjugated Antibody

Mouse Endoglin/CD105 PE-conjugated Antibody

Catalog #: FAB1320P
4 Citations Datasheet / COA / SDS

Discontinued Product

FAB1320P has been discontinued.
View all Endoglin/CD105 products.
Detection of Endoglin/CD105 in MS‑1 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endoglin/CD105 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endoglin/CD105 in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin.
Source
Monoclonal Rat IgG2A Clone # 209701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
Glu21-Gly581 (predicted)
Accession # NP_031958
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Endoglin/CD105 antibody in MS-1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Endoglin/CD105 in MS‑1 Mouse Cell Line by Flow Cytometry. MS-1 mouse pancreatic islet endothelial cell line was stained with Rat Anti-Mouse Endoglin/CD105 PE-conjugated Monoclonal Antibody (Catalog # FAB1320P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Endoglin/CD105

Endoglin (also known as CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts expressed on hematopoietic, mesenchymal, and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with Activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5 but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to anti-angiogenic activity (15).

References
  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat); 397096 (Porcine)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Mouse Endoglin/CD105 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tissue-Resident PDGFR&alpha+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner
    Authors: MP Santini, D Malide, G Hoffman, G Pandey, V D'Escamard, A Nomura-Kit, I Rovira, H Kataoka, J Ochando, RP Harvey, T Finkel, JC Kovacic
    Cell Rep, 2020-01-14;30(2):555-570.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging
    Authors: Chih-Lung Chen, Haosen Zhang, Qing Ye, Wen-Yuan Hsieh, T. Kevin Hitchens, Hsin-Hsin Shen et al.
    Molecular Imaging and Biology
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
    Authors: Ninagawa N, Murakami R, Isobe E, Tanaka Y, Nakagawa H, Torihashi S
    Differentiation, 2011-08-19;82(3):153-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  4. Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast code.
    Authors: Pimanda JE, Chan WY, Wilson NK, Smith AM, Kinston S, Knezevic K, Janes ME, Landry JR, Kolb-Kokocinski A, Frampton J, Tannahill D, Ottersbach K, Follows GA, Lacaud G, Kouskoff V, Gottgens B
    Blood, 2008-09-19;112(12):4512-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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