Mouse Ephrin-B2 Biotinylated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Ephrin-B2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Ephrin-A5 is observed and less than 1% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2
    Arg27-Ala227
    Accession # AAA82934
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Ephrin-B2 Fc Chimera (Catalog # 496-EB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Ephrin-B2 in Rat Brain. Ephrin-B2 was detected in perfusion fixed frozen sections of rat brain (trigeminal ganglia) using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
Ephrin‑B2 in Rat Hippocampal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using Goat Anti-Mouse Ephrin‑B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

  • References:
    1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
    2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  • Entrez Gene IDs:
    1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
  • Alternate Names:
    EFNB2; ELF-2; EphrinB2; Ephrin-B2; Htk-L; LERK-5; NLERK-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015;6(0):6625.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Emerging pulmonary vasculature lacks fate specification.
    Authors: Schwarz MA, Caldwell L, Cafasso D, Zheng H
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(1):L71-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse Ephrin-B2 Biotinylated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information