Epimorphin/Syntaxin 2 in Embryonic Mouse Eye Cup. |
Epimorphin/Syntaxin 2 was detected in immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.) using 8 µg/mL Rat Anti-Mouse Epimorphin/Syntaxin 2 Monoclonal Antibody (Catalog # MAB2568) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Epimorphin, also known as syntaxin 2, is a member of the highly conserved syntaxin family. It is a type IV integral membrane protein with a C-terminal membrane anchor and an N-terminal functional domain. Epimorphin can assume membrane topology with the functional domain localized extracellularly or to the cytoplasmic face. Extracellular Epimorphin functions in morphogenesis while intracellular Epimorphin functions as a vesicle fusion protein. Mouse Epimorphin functional domain shares 97% and 91% amino acid sequence homology with rat and human Epimorphin, respectively.
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