Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 340236
View all ESAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ESAM
Gln30-Ser248 (predicted)
Accession # Q925F2

Specificity

Detects mouse ESAM in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse ESAM Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver, thymus, and intestine

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ESAM

ESAM (Endothelial Cell-Specific Adhesion Molecule) is a 55 kDa type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% amino acid sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.

Long Name

Endothelial Cell Adhesion Molecule

Alternate Names

2310008D05Rik, endothelial cell adhesion molecule, endothelial cell-selective adhesion molecule, HUEL (C4orf1)-interacting protein, LP4791 protein, W117m

Entrez Gene IDs

90952 (Human); 69524 (Mouse)

Gene Symbol

ESAM

UniProt

Q925F2

Additional ESAM Products

Product Documents for Mouse ESAM Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ESAM Antibody

For research use only

Citations for Mouse ESAM Antibody

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Protocols

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FAQs

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