Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FGF acidic/FGF1
Phe16-Asp155
Accession # NP_034327

Specificity

Detects mouse FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human FGF acidic is observed, and less than 1% cross-reactivity with recombinant mouse (rm) FGF7, rmFGF21, and rmFGF23 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse FGF acidic/FGF1 Antibody

Detection of Mouse FGF acidic/FGF1 antibody by Western Blot.

Detection of Mouse FGF acidic/FGF1 by Western Blot.

Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FGF acidic/FGF1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Mouse FGF acidic/FGF1 Antibody.

Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Mouse FGF acidic/FGF1 Antibody.

Recombinant Mouse FGF acidic/FGF1 (Catalog # 4686-FA) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF acidic/FGF1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse FGF acidic/FGF1 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4686). The ND50 is typically 0.1-0.6 µg/mL.

Applications for Mouse FGF acidic/FGF1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse heart tissue and mouse kidney tissue

Neutralization

Measured by its ability to neutralize FGF acidic/FGF1-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.The Neutralization Dose (ND50) is typically 0.1-0.6 μg/mL in the presence of 1 ng/mL Recombinant Mouse FGF acidic/FGF1.

Reviewed Applications

Read 1 review rated 4 using AF4686 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Mouse FGF acidic shares 52% amino acid (aa) sequence identity with FGF basic and 15%-35% with other mouse FGFs. It shares 91%, 96%, 94%, and 100% aa sequence identity with bovine, human, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). The secreted FGF acidic is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulating FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10-12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

References

  1. Madiai, F. et al. (1996) Gene 179:231.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  11. Imamura, T. et al. (1990) Science 249:1567.
  12. Wesche, J. et al. (2005) Biochemistry 44:6071.
  13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  15. Bouleau, S. et al. (2005) Oncogene 24:7839.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Entrez Gene IDs

2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

Gene Symbol

FGF1

UniProt

NP_034327 (Mouse)

Additional FGF acidic/FGF1 Products

Product Documents for Mouse FGF acidic/FGF1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FGF acidic/FGF1 Antibody

For research use only

Citations for Mouse FGF acidic/FGF1 Antibody

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  • Mouse FGF acidic Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 04/18/2019
    Mouse FGF acidic/FGF1 Antibody AF4686

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