Mouse Follistatin-like 1/FSTL1 Antibody

Catalog # Availability Size / Price Qty
AF1738
AF1738-SP
Detection of Mouse Follistatin-like 1/FSTL1 by Immunohistochemistry
3 Images
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews

Mouse Follistatin-like 1/FSTL1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Follistatin-like 1/FSTL1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
Glu19-Ile306
Accession # Q62356
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Follistatin-like 1/FSTL1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of Mouse Follistatin-like 1/FSTL1 by Immunohistochemistry View Larger

Detection of Mouse Follistatin-like 1/FSTL1 by Immunohistochemistry Proliferation defects in Fstl1-/- ureteric epithelial cells.(A-D) Analysis of cell proliferation on transverse sections of wild-type (A, C) and Fstl1-/- (B, D) ureters by BrdU incorporation at E15.5 (A, B) and E16.5 (distal segment) (C, D). Arrows point to representative proliferating cells. (E) Quantification of cell proliferation by BrdU labeling index. In E15.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.49 for mesenchymal inner layer (n = 20). In distal segment of E16.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.59 for mesenchymal inner layer (n = 20). (F, G) BrdU incorporation analysis of epithelial cell proliferation on transverse section of cultured E15.0 ureters treated with conditioned media transfected either with pcDNA3.1 vector (Mock) (F) or an Fstl1 overexpress plasmid (G). Arrows point to representative proliferating cells. (H) HEK293 cells were transfected with a plasmid expressing mouse Fstl1 protein or pcDNA3.1 (mock). Western blot analysis using an anti-Fstl1 antibody indicated that Fstl1 was overexpressed in both the cultured media and cell pellet. (I) Quantification of cell proliferation by BrdU labeling index in the cultured E15.0 ureteric epithelium. p<0.005 (n  = 7). Scale bar: (A-D, F-G) 20µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0032554), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Follistatin-like 1/FSTL1 by Western Blot View Larger

Detection of Human Follistatin-like 1/FSTL1 by Western Blot Proliferation defects in Fstl1-/- ureteric epithelial cells.(A-D) Analysis of cell proliferation on transverse sections of wild-type (A, C) and Fstl1-/- (B, D) ureters by BrdU incorporation at E15.5 (A, B) and E16.5 (distal segment) (C, D). Arrows point to representative proliferating cells. (E) Quantification of cell proliferation by BrdU labeling index. In E15.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.49 for mesenchymal inner layer (n = 20). In distal segment of E16.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.59 for mesenchymal inner layer (n = 20). (F, G) BrdU incorporation analysis of epithelial cell proliferation on transverse section of cultured E15.0 ureters treated with conditioned media transfected either with pcDNA3.1 vector (Mock) (F) or an Fstl1 overexpress plasmid (G). Arrows point to representative proliferating cells. (H) HEK293 cells were transfected with a plasmid expressing mouse Fstl1 protein or pcDNA3.1 (mock). Western blot analysis using an anti-Fstl1 antibody indicated that Fstl1 was overexpressed in both the cultured media and cell pellet. (I) Quantification of cell proliferation by BrdU labeling index in the cultured E15.0 ureteric epithelium. p<0.005 (n  = 7). Scale bar: (A-D, F-G) 20µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0032554), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown Validated Detection of Mouse Follistatin-like 1/FSTL1 by Knockdown Validated View Larger

Detection of Mouse Follistatin-like 1/FSTL1 by Knockdown Validated Endogenous Fstl1 does not contribute to TGF‐ beta 1/Smad 2/3 signaling or myofibroblast transdifferentiationFstl1 ablation by siRNA Fstl1 (6 pmol) in NRCFbs. siRNA Fstl1 or siRNA non‐targeting negative control was transfected to NRCFbs by lipofectamine RNAimax for 12 h, followed by stimulation of recombinant TGF‐ beta 1 (10 ng/ml) for 24 h. Fstl1 protein expression in cell lysate was analyzed by immunoblotting.Efficiency of Fstl1 gene ablation by siRNA Fstl1 (6 pmol) in NRCFbs. siRNA Fstl1 or siRNA non‐targeting negative control was transfected to NRCFbs by lipofectamine RNAimax for 42 h. mRNA transcripts of Fstl1 and GAPDH were assessed by qPCR. The amplification plots for Fstl1 are shown. The Ct values for Fstl1 in siRNA control and siRNA Fstl1 are 18.17 ± 0.053 and 24.76 ± 0.420, respectively (n = 3 for each sample group).Effect of endogenous Fstl1 protein on TGF‐ beta 1‐induced Smad2/3 signaling pathway in NRCFbs. Endogenous Fstl1 protein was ablated by transfecting siRNA Fstl1 to NRCFbs. siRNA negative control was used for control. Following FBS starvation for 24 h, cells were stimulated with recombinant TGF‐ beta 1 protein (2 ng/ml) for 15 min. Phosphorylation of Smad2 and Smad3 was assessed by immunoblotting. Error bars represent mean ± SEM (n = 3 for each group). Statistical analysis was performed by ordinary one‐way ANOVA. Post hoc test was performed by Dunnett's T3 test for Smad2 and Tukey's test for Smad3. Two independent experiments were performed.Ablation of endogenous Fstl1 does not affect markers of myofibroblast differentiation or ECM protein synthesis. Endogenous Fstl1 was ablated by siRNA, and following FBS depletion for 24 h, cells were stimulated with recombinant Fstl1 protein (50 ng/ml) for 24 h. alpha ‐SMA protein expression in cell lysate and fibronectin and collagen I protein in the cultured media were assessed by immunoblotting. Tubulin was used as an internal control. Error bars represent mean ± SEM (n = 4 for each group). Statistical analysis was performed by ordinary one‐way ANOVA and Tukey's test. Two independent experiments were performed.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27234440), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 1/FSTL1

Mouse FSTL1, also known as FSL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Entrez Gene IDs
11167 (Human); 14314 (Mouse); 79210 (Rat)
Alternate Names
Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

Product Datasheets

You must select a language.

x

Citations for Mouse Follistatin-like 1/FSTL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Deficiency of Follistatin-Like Protein 1 Accelerates the Growth of Breast Cancer Cells at Lung Metastatic Sites
    Authors: Ying Zhang, Xiaozhou Xu, Ying Yang, Jie Ma, Lulu Wang, Xiangzhi Meng et al.
    Journal of Breast Cancer
  2. Follistatin-Like-1 (FSTL1) Is a Fibroblast-Derived Growth Factor That Contributes to Progression of Chronic Kidney Disease
    Authors: Nicholas A. Maksimowski, Xuewen Song, Eun Hui Bae, Heather Reich, Rohan John, York Pei et al.
    International Journal of Molecular Sciences
  3. Follistatin-like protein 1 increases transepithelial resistance in kidney epithelial cells through Akt signaling
    Authors: F Chen, Q Hu, H Huang, B Chen, Y Xia, W Liu
    Mol Med Rep, 2017-07-31;0(0):.
  4. Follistatin-like protein 1 is a mesenchyme-derived inflammatory protein and may represent a biomarker for systemic-onset juvenile rheumatoid arthritis
    Authors: David C. Wilson, Anthony D. Marinov, Harry C. Blair, Daniel S. Bushnell, Susan D. Thompson, Yury Chaly et al.
    Arthritis & Rheumatism
  5. FSTL1 Suppresses Triple-Negative Breast Cancer Lung Metastasis by Inhibiting M2-like Tumor-Associated Macrophage Recruitment toward the Lungs
    Authors: Ying Yang, Tao Lu, Xiaowei Jia, Yan Gao
    Diagnostics (Basel)
  6. Follistatin-like Protein 1 Promotes Arthritis by Enhancing Inflammatory Cytokine/Chemokine Expression
    Authors: Yury Chaly, Anthony D. Marinov, Leif Oxburgh, Daniel S. Bushnell, Raphael Hirsch
    Arthritis & Rheumatism
  7. Follistatin-like 1 deficiency impairs T cell development to promote lung metastasis of triple negative breast cancer
    Authors: Jie Ma, Ying Yang, Lulu Wang, Xiaowei Jia, Tao Lu, Yiyan Zeng et al.
    Aging (Albany NY)
  8. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
    Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
    The Journal of Biological Chemistry, 2022-05-04;0(0):102010.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Immunoprecipitation, Western Blot
  9. Brown adipose tissue involution associated with progressive restriction in progenitor competence
    Authors: Z Huang, Z Zhang, Z Moazzami, R Heck, P Hu, H Nanda, K Ren, Z Sun, A Bartolomuc, Y Gao, D Chung, W Zhu, S Shen, HB Ruan
    Cell Reports, 2022-04-12;39(2):110575.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  10. Ups and downs: the PPARgamma/p-PPARgamma seesaw of follistatin-like 1 and integrin receptor signaling in adipogenesis
    Authors: D Fang, X Shi, X Jia, C Yang, L Wang, B Du, T Lu, L Shan, Y Gao
    Molecular Metabolism, 2021-11-20;0(0):101400.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury
    Authors: D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi
    Sci Rep, 2019-11-13;9(1):16736.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  12. Profiling proliferative cells and their progeny in damaged murine hearts
    Authors: K Kretzschma, Y Post, M Bannier-Hé, A Mattiotti, J Drost, O Basak, VSW Li, M van den Bo, QD Gunst, D Versteeg, L Kooijman, S van der El, JH van Es, E van Rooij, MJB van den Ho, H Clevers
    Proc. Natl. Acad. Sci. U.S.A., 2018-12-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  13. Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3
    Authors: S Yamada, N Itoh, T Nagai, T Nakai, D Ibi, A Nakajima, T Nabeshima, K Yamada
    J Neuroinflammation, 2018-10-22;15(1):295.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC, Western Blot
  14. Follistatin like-1 (Fstl1) is required for the normal formation of lung airway and vascular smooth muscle at birth
    Authors: X Liu, Y Liu, X Li, J Zhao, Y Geng, W Ning
    PLoS ONE, 2017-06-02;12(6):e0177899.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Follistatin-like 1 promotes cardiac fibroblast activation and protects the heart from rupture
    EMBO Mol Med, 2016-08-01;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  16. Follistatin-like protein 1 enhances NLRP3 inflammasome-mediated IL-1beta secretion from monocytes and macrophages.
    Authors: Chaly Y, Fu Y, Marinov A, Hostager B, Yan W, Campfield B, Kellum J, Bushnell D, Wang Y, Vockley J, Hirsch R
    Eur J Immunol, 2014-02-20;44(5):1467-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Fstl1 antagonizes BMP signaling and regulates ureter development.
    Authors: Xu J, Qi X, Gong J
    PLoS ONE, 2012-04-02;7(4):e32554.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
    Authors: Ippagunta SK, Malireddi RK, Shaw PJ
    Nat. Immunol., 2011-09-04;12(10):1010-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Follistatin-like protein-1 is a novel proinflammatory molecule.
    Authors: Miyamae T, Marinov AD, Sowders D, Wilson DC, Devlin J, Boudreau R, Robbins P, Hirsch R
    J. Immunol., 2006-10-01;177(7):4758-62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Lactucin, a Bitter Sesquiterpene from Cichorium intybus, Inhibits Cancer Cell Proliferation by Downregulating the MAPK and Central Carbon Metabolism Pathway
    Authors: Imam KMSU, Tian Y, Xin F et al.
    Molecules (Basel, Switzerland)

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Follistatin-like 1/FSTL1 Antibody

There are currently no reviews for this product. Be the first to review Mouse Follistatin-like 1/FSTL1 Antibody and earn rewards!

Have you used Mouse Follistatin-like 1/FSTL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review