Detection of FPRP in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3‑L1 mouse embryonic fibroblast adipose‑like cell line was stained with Sheep Anti-Mouse FPRP Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4495, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Prostaglandin F2-alpha Receptor Regulatory Protein
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