FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Mouse FPRP/PTGFRN Antibody
R&D Systems | Catalog # AF4495
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FPRP/PTGFRN
Arg22-Pro832
Accession # NP_035327
Arg22-Pro832
Accession # NP_035327
Specificity
Detects mouse FPRP/PTGFRN in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse FPRP/PTGFRN Antibody
Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4495, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Northern-Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Applications for Mouse FPRP/PTGFRN Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse FPRP/PTGFRN
Sample: Recombinant Mouse FPRP/PTGFRN
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FPRP/PTGFRN
Long Name
Prostaglandin F2-alpha Receptor Regulatory Protein
Alternate Names
CD315, CD9P-1, PTGFRN
Gene Symbol
PTGFRN
UniProt
Additional FPRP/PTGFRN Products
Product Documents for Mouse FPRP/PTGFRN Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse FPRP/PTGFRN Antibody
For research use only
Related Research Areas
Citations for Mouse FPRP/PTGFRN Antibody
Powered by Bioz
Customer Reviews for Mouse FPRP/PTGFRN Antibody
There are currently no reviews for this product. Be the first to review Mouse FPRP/PTGFRN Antibody and earn rewards!
Have you used Mouse FPRP/PTGFRN Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars