Detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.
Monoclonal Rat IgG2A Clone # 759821
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Gastrokine 1 Tyr38-Tyr201 Accession # NP_079742
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Mouse and Rat Gastrokine 1 by Western Blot.
Western blot shows lysates of mouse stomach tissue and rat stomach tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Gastrokine 1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Gastrokine 1 in Mouse Intestine.
Gastrokine 1 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gastrokine 1
Gastrokine 1 (GKN1; also CA11 and AMP-18) is a 17-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine 1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine 1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine 1 is 166 amino acids (aa) in length (aa 36-201 of SwissProt accession Q9CR36 or aa 19-184 of accession NP_079742). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201 (accession Q9CR36), mouse gastrokine 1 shares 65% and 92% aa sequence identity with human and rat gastrokine 1, respectively.
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