Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 759821
View all Gastrokine 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Gastrokine 1
Tyr38-Tyr201
Accession # NP_079742

Specificity

Detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Gastrokine 1 Antibody

Detection of Mouse and Rat Gastrokine 1 antibody by Western Blot.

Detection of Mouse and Rat Gastrokine 1 by Western Blot.

Western blot shows lysates of mouse stomach tissue and rat stomach tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Gastrokine 1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Gastrokine 1 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr).

Gastrokine 1 in Mouse Intestine.

Gastrokine 1 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Gastrokine 1 Monoclonal Antibody (Catalog # MAB7287) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Gastrokine 1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Western Blot

1 µg/mL
Sample: Mouse stomach tissue and rat stomach tissue

Reviewed Applications

Read 1 review rated 5 using MAB7287 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gastrokine 1

Gastrokine 1 (GKN1; also CA11 and AMP-18) is a 17-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine 1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine 1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine 1 is 166 amino acids (aa) in length (aa 36-201 of SwissProt accession Q9CR36 or aa 19-184 of accession NP_079742). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201 (accession Q9CR36), mouse gastrokine 1 shares 65% and 92% aa sequence identity with human and rat gastrokine 1, respectively.

Long Name

Take out Column D name

Alternate Names

AMP18, BRICD1, CA11, FOV, Foveolin, GKN1

Entrez Gene IDs

56287 (Human); 66283 (Mouse); 297418 (Rat)

Gene Symbol

GKN1

UniProt

NP_079742

Additional Gastrokine 1 Products

Product Documents for Mouse Gastrokine 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Gastrokine 1 Antibody

For research use only

Citations for Mouse Gastrokine 1 Antibody

Customer Reviews for Mouse Gastrokine 1 Antibody (1)

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  • Mouse Gastrokine 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Intestine
    Species: Mouse
    Verified Customer | Posted 03/06/2022
    Mouse Gastrokine 1 Antibody MAB7287

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Protocols

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