Mouse GDF-5/BMP-14 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF853

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Product Details
FAQs
Supplemental Products
Reviews (1)

Mouse GDF-5/BMP-14 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF‑5/BMP‑14 in Western blots. In Western blot, approximately 20% cross-reactivity with recombinant mouse (rm) GDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-8, and rmGDF-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF‑5/BMP‑14
Ala376-Arg495
Accession # P43027
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GDF‑5 (Catalog # 853-G5)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.
Long Name
Growth Differentiation Factor 5
Entrez Gene IDs
8200 (Human); 14563 (Mouse)
Alternate Names
BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse GDF-5/BMP-14 Biotinylated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse GDF-5/BMP-14 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse GDF-5/BMP-14 Biotinylated Antibody
By Anonymous on 10/26/2021
Application: B/N Sample Tested: Recombinant protein Species: Mouse