Mouse GDF-5/BMP-14 Biotinylated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse GDF‑5/BMP‑14 in Western blots. In Western blot, approximately 20% cross-reactivity with recombinant mouse (rm) GDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-8, and rmGDF-9 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse GDF‑5/BMP‑14
    Ala376-Arg495
    Accession # P43027
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse GDF‑5 (Catalog # 853-G5)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

  • References:
    1. Storm, E.E. et al. (1994) Nature 368:639.
    2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
    3. Francis-West, P.H. et al. (1999) Development 126:1035.
    4. Massague, J. et al. (2000) Genes and Dev. 14:627.
    5. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
    6. Inada, M. et al. (1996) BBRC 222:317.
  • Long Name:
    Growth Differentiation Factor 5
  • Entrez Gene IDs:
    8200 (Human); 14563 (Mouse)
  • Alternate Names:
    BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2
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Isotype Controls
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