Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant mouse GDF‑5/BMP‑14
Ala376-Arg495
Accession # P43027
Ala376-Arg495
Accession # P43027
Specificity
Detects mouse GDF‑5/BMP‑14 in Western blots. In Western blot, approximately 20% cross-reactivity with recombinant mouse (rm) GDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-8, and rmGDF-9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse GDF‑5/BMP‑14 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse GDF‑5 (Catalog # 853-G5)
Sample: Recombinant Mouse GDF‑5 (Catalog # 853-G5)
Reviewed Applications
Read 1 review rated 5 using BAF853 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GDF-5/BMP-14
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Francis-West, P.H. et al. (1999) Development 126:1035.
- Massague, J. et al. (2000) Genes and Dev. 14:627.
- Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
- Inada, M. et al. (1996) BBRC 222:317.
Long Name
Growth Differentiation Factor 5
Alternate Names
BMP-14, CDMP-1, GDF5
Gene Symbol
GDF5
UniProt
Additional GDF-5/BMP-14 Products
Product Documents for Mouse GDF‑5/BMP‑14 Biotinylated Antibody
Product Specific Notices for Mouse GDF‑5/BMP‑14 Biotinylated Antibody
For research use only
Related Research Areas
Customer Reviews for Mouse GDF‑5/BMP‑14 Biotinylated Antibody (1)
5 out of 5
1 Customer Rating
Have you used Mouse GDF‑5/BMP‑14 Biotinylated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Block/NeutralizeSample Tested: Recombinant proteinSpecies: MouseVerified Customer | Posted 10/26/2021
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
