Mouse GDF-5/BMP-14 Biotinylated Antibody

Name: Mouse GDF-5/BMP-14 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF853
Rating: 5 (1 reviews)

Catalog #: BAF853 1 Review Datasheet / COA / SDS

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BAF853

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GDF-5/BMP-14 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GDF‑5/BMP‑14 in Western blots. In Western blot, approximately 20% cross-reactivity with recombinant mouse (rm) GDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-8, and rmGDF-9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse GDF‑5/BMP‑14
Ala376-Arg495
Accession # P43027

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse GDF‑5 (Catalog # 853-G5)

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

Storm, E.E. et al. (1994) Nature 368:639.
Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
Francis-West, P.H. et al. (1999) Development 126:1035.
Massague, J. et al. (2000) Genes and Dev. 14:627.
Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Inada, M. et al. (1996) BBRC 222:317.

Long Name

Growth Differentiation Factor 5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Alternate Names

BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2