Mouse GITR/TNFRSF18 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse GITR/TNFRSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, recombinant mouse (rm) CD27, rmCD30, rmEDAR, rmFas, rhGITR, rhHVEM, rmRANK, rhTROY, and rmTNF R1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 108619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse GITR/TNFRSF18
    Met1-His153
    Accession # O35714
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse GITR/TNFRSF18 Fc Chimera (Catalog # 524-GR)
    under non-reducing conditions only
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GITR/TNFRSF18
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a transmembrane TNF receptor superfamily member that functions in immune regulation. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes, and it is upregulated on antigen-activated conventional CD4+ and CD8+ T cells. GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells. It also induces the proliferation of Treg but inhibits the ability of Treg to suppress immune responses. This can result in the development of autoimmunity, increased tumor cell killing by effector T cells, and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease. GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
Sample Type
  1. Prevention of cytotoxic T lymphocyte responses to factor IX-expressing hepatocytes by gene transfer-induced regulatory T cells.
    Authors: Dobrzynski E, Fitzgerald JC, Cao O, Mingozzi F, Wang L, Herzog RW
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(12):4592-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Protection against autoimmunity in nonlymphopenic hosts by CD4+ CD25+ regulatory T cells is antigen-specific and requires IL-10 and TGF-beta.
    Authors: Huang X, Zhu J, Yang Y
    J. Immunol., 2005;175(7):4283-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression.
    Authors: Ji HB, Liao G, Faubion WA, Abadia-Molina AC, Cozzo C, Laroux FS, Caton A, Terhorst C
    J. Immunol., 2004;172(10):5823-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression.
    Authors: Ji HB, Liao G, Faubion WA, Abadia-Molina AC, Cozzo C, Laroux FS, Caton A, Terhorst C
    J. Immunol., 2004;172(10):5823-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. An essential role for Scurfin in CD4+CD25+ T regulatory cells.
    Authors: Khattri, Roli, Cox, Tom, Yasayko, Sue-Ann, Ramsdell, Fred
    Nat Immunol, 2003;4(4):337-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 51  
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Normal Rat IgG Control

Ctrl 6-001-A 5
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF005 3  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 1  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
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