Mouse GITR/TNFRSF18 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse GITR/TNFRSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, recombinant mouse (rm) CD27, rmCD30, rmEDAR, rmFas, rhGITR, rhHVEM, rmRANK, rhTROY, and rmTNF R1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 108619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse GITR/TNFRSF18
    Met1-His153
    Accession # O35714
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse GITR/TNFRSF18 Fc Chimera (Catalog # 524-GR)
    under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor; also named AITR) is a member of the co‑stimulatory subset of the TNF receptor superfamily (1, 2). In mouse, the GITR gene is composed of five exons and encodes multiple length isoforms that arise from alternate splicing. The “standard”, or first reported isoform is a type I transmembrane protein, 228 amino acids (aa) in length that contains a 19 aa signal sequence, a 134 aa extracellular region, a 21 aa transmembrane segment, and a 54 aa cytoplasmic domain. The extracellular region contains four potential N-linked glycosylation sites plus three cysteine-rich pseudorepeats of about 40 aa each (3, 4). The extracellular regions of mouse and human are 57% aa identical. The cytoplasmic domain has a P-x-Q/E-E motif that is known to associate with TRAF2. This is a common characteristic of TNFRSF members with co‑stimulatory functions (4). Three other mouse GITR isoforms (B, C and D) have been reported (5). All share the same N-terminal 101 of 134 aa in the extracellular region (including pseudorepeats #1, #2 and one-half of #3). Isoform D diverges at aa #101 and continues for another 12 aa for a total length of 113 aa. This is a naturally-occurring soluble form. Isoforms B and C show splicing in their cytoplasmic tails that creates cytoplasmic domains of 118 aa and 46 aa, respectively. In both the B and C isoforms, the TRAF2 binding site is spliced out, with a p56lck binding site inserted in isoform B (4). Given its membership in the TNFRSF, it likely functions as a trimer on the cell surface (2). GITR is predominantly expressed on CD4+CD25+ regulatory T cells (Treg) and naïve CD8+ and CD4+ CD25- T cells, where its expression is up-regulated after antigen-driven activation. GITR activation provides co‑stimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production (IL-2, IL-4, IFN-gamma ). On CD4+ CD25+ Treg cells, GITR activation provides co‑stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state (6‑8).

  • References:
    1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. 
    2. Croft, M. (2003) Nat. Rev. Immunol. 3:609. 
    3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
    4. Nocentini, G. et al. (2000) DNA Cell Biol. 19:205. 
    5. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
    6. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
    7. Ji, H. et al. (2004) J. Immunol. 172:5823.
    8. Stephens, G.L. et al. (2004) 173:5008.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Prevention of cytotoxic T lymphocyte responses to factor IX-expressing hepatocytes by gene transfer-induced regulatory T cells.
    Authors: Dobrzynski E, Fitzgerald JC, Cao O, Mingozzi F, Wang L, Herzog RW
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(12):4592-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Protection against autoimmunity in nonlymphopenic hosts by CD4+ CD25+ regulatory T cells is antigen-specific and requires IL-10 and TGF-beta.
    Authors: Huang X, Zhu J, Yang Y
    J. Immunol., 2005;175(7):4283-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression.
    Authors: Ji HB, Liao G, Faubion WA, Abadia-Molina AC, Cozzo C, Laroux FS, Caton A, Terhorst C
    J. Immunol., 2004;172(10):5823-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression.
    Authors: Ji HB, Liao G, Faubion WA, Abadia-Molina AC, Cozzo C, Laroux FS, Caton A, Terhorst C
    J. Immunol., 2004;172(10):5823-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. An essential role for Scurfin in CD4+CD25+ T regulatory cells.
    Authors: Khattri, Roli, Cox, Tom, Yasayko, Sue-Ann, Ramsdell, Fred
    Nat Immunol, 2003;4(4):337-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 71  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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APC-conjugated Antibody

Flow F0113 2  
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Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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