Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
Thr25-Gln273
Accession # Q3UP14

Specificity

Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Growth Hormone R/GHR Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

Long Name

Growth Hormone Receptor

Alternate Names

GHR

Entrez Gene IDs

2690 (Human); 14600 (Mouse); 25235 (Rat)

Gene Symbol

GHR

UniProt

Additional Growth Hormone R/GHR Products

Product Documents for Mouse Growth Hormone R/GHR Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Growth Hormone R/GHR Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Protocols

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