Mouse Growth Hormone R/GHR Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
    Thr25-Gln273
    Accession # Q3UP14
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Growth Hormone R/GHR Fc Chimera (Catalog # 1360-GR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Growth Hormone R/GHR in Mouse Liver. Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

  • References:
    1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
    2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
    3. Clark, R. (1997) Endocr. Rev. 18:157.
    4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
    5. Smith, W.C. et al. (1989) Mol. Endocrinol. 3:984.
    6. Edens, A. et al. (1994) Endocrinol. 135:2802.
    7. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
    8. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
  • Long Name:
    Growth Hormone Receptor
  • Entrez Gene IDs:
    2690 (Human); 14600 (Mouse); 25235 (Rat)
  • Alternate Names:
    GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Molecular interactions of EphA4, growth hormone receptor, Janus kinase 2, and signal transducer and activator of transcription 5B
    Authors: T Sawada, D Arai, X Jing, M Miyajima, SJ Frank, K Sakaguchi
    PLoS ONE, 2017;12(7):e0180785.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Growth hormone is increased in the lungs and enhances experimental lung metastasis of melanoma in DJ-1 KO mice
    BMC Cancer, 2016;16(1):871.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Hepatic growth hormone resistance after acute injury.
    Authors: Corrick, Ryan M, Li, Li, Frank, Stuart J, Messina, Joseph L
    Endocrinology, 2013;154(4):1577-88.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
    Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
    J. Exp. Med., 2009;206(4):819-31.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  5. Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression.
    Authors: Anzo M, Cobb LJ, Hwang DL, Mehta H, Said JW, Yakar S, LeRoith D, Cohen P
    Cancer Res., 2008;68(9):3342-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Isotype Controls
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Normal Goat IgG Control

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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