Mouse Growth Hormone R/GHR Antibody

Catalog # Availability Size / Price Qty
AF1360
AF1360-SP
Growth Hormone R/GHR in Mouse Liver.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Mouse Growth Hormone R/GHR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Growth Hormone R/GHR in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant rat GHR is observed and approximately 20% cross-reactivity with recombinant human GHR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
Thr25-Gln273
Accession # Q3UP14
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Growth Hormone R/GHR Fc Chimera (Catalog # 1360-GR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Growth Hormone R/GHR antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

Growth Hormone R/GHR in Mouse Liver. Growth Hormone R/GHR was detected in perfusion fixed frozen sections of mouse liver using Mouse Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

References
  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Smith, W.C. et al. (1989) Mol. Endocrinol. 3:984.
  6. Edens, A. et al. (1994) Endocrinol. 135:2802.
  7. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  8. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.
Long Name
Growth Hormone Receptor
Entrez Gene IDs
2690 (Human); 14600 (Mouse); 25235 (Rat)
Alternate Names
GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor

Product Datasheets

You must select a language.

x

Citations for Mouse Growth Hormone R/GHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Molecular interactions of EphA4, growth hormone receptor, Janus kinase 2, and signal transducer and activator of transcription 5B
    Authors: T Sawada, D Arai, X Jing, M Miyajima, SJ Frank, K Sakaguchi
    PLoS ONE, 2017-07-07;12(7):e0180785.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Growth hormone is increased in the lungs and enhances experimental lung metastasis of melanoma in DJ-1 KO mice
    BMC Cancer, 2016-11-08;16(1):871.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Hepatic growth hormone resistance after acute injury.
    Authors: Corrick, Ryan M, Li, Li, Frank, Stuart J, Messina, Joseph L
    Endocrinology, 2013-02-15;154(4):1577-88.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation.
    Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L
    J. Exp. Med., 2009-03-30;206(4):819-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  5. Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression.
    Authors: Anzo M, Cobb LJ, Hwang DL, Mehta H, Said JW, Yakar S, LeRoith D, Cohen P
    Cancer Res., 2008-05-01;68(9):3342-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Growth Hormone R/GHR Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse Growth Hormone R/GHR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse Growth Hormone R/GHR Antibody
By Anonymous on 04/18/2019
Application: WB Sample Tested: Liver tissue Species: Mouse