Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Growth Hormone R/GHR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
Thr25-Gln273
Accession # P16882

Specificity

Detects mouse Growth Hormone R/GHR in Western blots. In Western blots, approximately 30% cross‑reactivity with recombinant rat GHR is observed and 10% cross-reactivity with recombinant human GHR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Growth Hormone R Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney, liver, lung, and muscle

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Growth Hormone R/GHR Fc Chimera (Catalog # 1360-GR)

Reviewed Applications

Read 1 review rated 4 using BAF1360 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum
GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

References

  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Clark, R. (1997) Endocr. Rev. 18:157.
  4. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  5. Smith, W.C. et al. (1989) Mol. Endocrinol. 3:984.
  6. Edens, A. et al. (1994) Endocrinol. 135:2802.
  7. Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
  8. Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Long Name

Growth Hormone Receptor

Alternate Names

GHR

Entrez Gene IDs

2690 (Human); 14600 (Mouse); 25235 (Rat)

Gene Symbol

GHR

UniProt

P16882

Additional Growth Hormone R/GHR Products

Product Documents for Mouse Growth Hormone R Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Growth Hormone R Biotinylated Antibody

For research use only

Citations for Mouse Growth Hormone R Biotinylated Antibody

Customer Reviews for Mouse Growth Hormone R Biotinylated Antibody (1)

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  • Mouse Growth Hormone R Biotinylated Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: Bone Extracts and bone marrow
    Species: Mouse
    Verified Customer | Posted 11/08/2016
    Mouse Growth Hormone R Biotinylated Antibody BAF1360

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