Mouse Growth Hormone R Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1360
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse Growth Hormone R Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Growth Hormone R/GHR in Western blots. In Western blots, approximately 30% cross‑reactivity with recombinant rat GHR is observed and 10% cross-reactivity with recombinant human GHR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Growth Hormone R/GHR
Thr25-Gln273
Accession # P16882
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Growth Hormone R/GHR Fc Chimera (Catalog # 1360-GR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse kidney, liver, lung, and muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Mouse GHR cDNA encodes a 650 amino acid (aa) residue type I transmembrane protein with a 24 aa signal peptide, a 249 aa extracellular domain, a 24 aa transmembrane domain, and a 353 aa cytoplasmic domain (5). An alternatively spliced secreted isoform of mouse GHR also exists (6). This variant corresponds to the serum
GH‑binding protein. Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (7). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8).

Long Name
Growth Hormone Receptor
Entrez Gene IDs
2690 (Human); 14600 (Mouse); 25235 (Rat)
Alternate Names
GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor

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Citation for Mouse Growth Hormone R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Insertional gene activation by lentiviral and gammaretroviral vectors.
    Authors: Bokhoven M, Stephen SL, Knight S, Gevers EF, Robinson IC, Takeuchi Y, Collins MK
    J. Virol., 2008-10-22;83(1):283-94.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Flow Cytometry, Western Blot

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Mouse Growth Hormone R Biotinylated Antibody
By Adam Guess on 11/08/2016
Application: Flow Sample Tested: Bone Extracts,bone marrow Species: Mouse