Mouse Haptoglobin Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Haptoglobin by Western Blot. Western blot shows samples of purified mouse Haptoglobin (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4409) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Haptoglobin was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Haptoglobin is a 45‑48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin. Mouse (pro)haptoglobin shares 90% and 80% aa identity with rat and human (pro)haptoglobin, respectively.
Citation for Mouse Haptoglobin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease
Authors: S Krishnamoo, B Pace, D Gupta, S Sturtevant, B Li, L Makala, J Brittain, N Moore, BF Vieira, T Thullen, I Stone, H Li, WE Hobbs, DR Light
JCI Insight, 2017;2(20):.
Sample Types: Tissue Homogenates
Applications: Western Blot
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