Mouse HVEM/TNFRSF14 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2516-100
MAB2516-SP
Detection  of HVEM/TNFRSF14 in Mouse Splenocytes by Flow Cytometry.
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Mouse HVEM/TNFRSF14 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HVEM/TNFRSF14 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2024B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse HVEM/TNFRSF14
Gln39-Val207
Accession # NP_849262
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of HVEM/TNFRSF14 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of HVEM/TNFRSF14 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rabbit Anti-Mouse HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB2516, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection  of HVEM/TNFRSF14 in CHO Chinese Hamster Cell Line Transfected with Mouse  HVEM  by Flow Cytometry. View Larger

Detection of HVEM/TNFRSF14 in CHO Chinese Hamster Cell Line Transfected with Mouse HVEM by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse HVEM was stained with Rabbit Anti-Mouse HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB2516, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator) is a type I membrane protein that is TNF receptor superfamily member 14 (TNFRSF14) (1). The mouse HVEM cDNA encodes a 275 amino acid (aa) protein. It contains a 36 aa signal peptide, a 170 aa extracellular domain with three cysteine rich domains (CRD), a 24 aa transmembrane region and a 45 aa cytoplasmic tail with a TRAF interaction domain (1). HVEM expression is highest on naïve, memory and regulatory T cells, but declines during T cell activation (2, 3). It is present at low levels on most resting leukocytes (4). HVEM is a receptor for the IGSF member BTLA (B and T lymphocyte attenuator), CD160, and the TNF family ligand LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) (2, 9). HVEM and BTLA are constitutively expressed on T cells, while LIGHT is generally considered to be inducible upon TCR activation. In the absence of activation, HVEM and BTLA interact monomerically, either in cis, or in trans. A same cell (or cis) interaction likely promotes general cell survival, while a between cell (or trans) interaction promotes a state of lymphocyte inactivity through the BTLA cytoplasmic domain. Following T cell activation, LIGHT appears and disrupts existing HVEM-BTLA bonds. A LIGHT-HVEM trimer now forms in trans, initiating HVEM-mediated NF kappa B signaling and a proinflammatory response (10). BTLA and LIGHT interactions are not mutually exclusive, but BTLA appears dominant (4, 6, 7). The herpesvirus envelope glycoprotein gD, which binds HVEM CRD1 to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (1, 6, 7, 9). Human, but not mouse, HVEM can also bind lymphotoxin a within CRD2 3 (9, 11). Graft‑vs‑host disease and Th1 type intestinal inflammation can be ameliorated by interrupting T cell LIGHT/HVEM interactions, while disruption of BTLA/HVEM interaction promotes intestinal inflammation (12-14). Mouse HVEM ECD shares 89% and 53% aa identity with rat and human HVEM, respectively. Mouse HVEM can recognize human BTLA and LIGHT, but human HVEM does not recognize mouse ligands (2, 11).

References
  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Sedy, J.R. et al. (2005) Nat. Immunol. 6:90.
  3. Tao, R. et al. (2008) J. Immunol. 180:6649.
  4. Wang, Y. et al. (2005) J. Clin. Invest. 115:711.
  5. Nelson, C.A. et al. (2008) J. Immunol. 180:940.
  6. Gonzales, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Cai, G. et al. (2008) Nat. Immunol. 9:176.
  9. Mauri, D.N. et al. (1998) Immunity 8:21.
  10. Ware, C.F. (2008) Immunol. Rev. 223:186.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Xu, Y. et al. (2007) Blood 109:4097.
  13. Wang, J. et al. (2005) J. Immunol. 174:8173.
  14. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
Long Name
Herpesvirus Entry Mediator
Entrez Gene IDs
8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names
ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2

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