Mouse ICAM-1/CD54 Biotinylated Antibody

Catalog # Availability Size / Price Qty
ICAM‑1/CD54 in Human Kidney.
1 Image
Product Details
Citations (3)
Supplemental Products

Mouse ICAM-1/CD54 Biotinylated Antibody Summary

Species Reactivity
Detects mouse ICAM‑1/CD54 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross‑reactivity with recombinant human ICAM-1, recombinant rat ICAM-1, and recombinant mouse ICAM-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑1/CD54
Accession # Q3U8M7
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)
2-10 µg/mL
See below

Mouse ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse ICAM‑1/CD54 Antibody (Catalog # MAB796)

Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF (Catalog # 796-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ICAM-1/CD54 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

ICAM‑1/CD54 in Human Kidney. ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

You must select a language.


Citations for Mouse ICAM-1/CD54 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Investigating the Role of Endothelial Glycogen Synthase Kinase3?/? in Atherogenesis in Low Density Lipoprotein Receptor Knockout Mice
    Authors: Mastrogiacomo L, Werstuck GH
    International journal of molecular sciences
  2. Mycobacterium bovis Bacillus Calmette–Guérin–Infected Dendritic Cells Induce TNF-alpha –Dependent Cell Cluster Formation That Promotes Bacterial Dissemination through an In Vitro Model of the Blood–Brain Barrier
    Authors: Trey E. Gilpin, Fruzsina R. Walter, Melinda Herbath, Matyas Sandor, Zsuzsanna Fabry
    The Journal of Immunology
  3. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse ICAM-1/CD54 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse ICAM-1/CD54 Biotinylated Antibody and earn rewards!

Have you used Mouse ICAM-1/CD54 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review