Mouse ICAM-1/CD54 Antibody

(28 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54
    Gln28-Asn485
    Accession # Q3U8M7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Mouse ICAM-1 Fc Chimera (Catalog # 796-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
ICAM‑1/CD54 in Mouse Testis. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

  • References:
    1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
    2. Siu, G. et al. (1989) J. Immunol. 143:3813.
    3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
    Authors: CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao
    Sci Rep, 2017;7(1):717.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Epidermal RAF prevents allergic skin disease
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  4. Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
    Authors: Goh Q, Dearth C, Corbett J, Pierre P, Chadee D, Pizza F
    Exp Cell Res, 2015;331(2):292-308.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  5. Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
    Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
    J Immunol, 2015;194(11):5061-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
    Authors: Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V
    PLoS ONE, 2015;10(3):e0120728.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015;10(3):e0119661.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
    Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P
    PLoS ONE, 2014;9(1):e87098.
    Species: Hamster
    Sample Type: Cell Lysates
    Application: WB
  9. CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
    Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
    PLoS ONE, 2013;8(12):e84479.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response.
    Authors: Dearth C, Goh Q, Marino J, Cicinelli P, Torres-Palsa M, Pierre P, Worth R, Pizza F
    PLoS ONE, 2013;8(3):e58486.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Peptide inhibitor of NF-kappaB translocation ameliorates experimental atherosclerosis.
    Authors: Mallavia B, Recio C, Oguiza A, Ortiz-Munoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C
    Am J Pathol, 2013;182(5):1910-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fluorescence
  12. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  13. Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation.
    Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
    Am. J. Pathol., 2011;179(1):125-33.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
    Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
    Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009;39(1):85-95.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  17. Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency.
    Authors: Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K
    J. Cell. Physiol., 2009;220(2):508-14.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  18. Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
    Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
    Kidney Int., 2009;75(1):52-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance.
    Authors: Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW
    Arterioscler. Thromb. Vasc. Biol., 2008;28(11):1982-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  20. PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms.
    Authors: Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R
    Brain Res., 2008;1244(0):164-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  21. Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.
    Authors: Casos K, Saiz MP, Ruiz-Sanz JI, Mitjavila MT
    Atherosclerosis, 2008;201(2):306-17.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  22. Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
    Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
    Immunity, 2007;27(2):296-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  23. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007;100(8):1226-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
    Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
    Circulation, 2007;117(3):421-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
    Authors: Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC
    Circ. Res., 2007;101(10):1001-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  26. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  27. Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.
    Authors: Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA
    Br. J. Pharmacol., 2006;149(5):591-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  28. Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
    Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 28 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse ICAM-1/CD54 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information