Mouse ICAM-1/CD54 Antibody

Catalog # Availability Size / Price Qty
AF796
AF796-SP
ICAM‑1/CD54 in Mouse Testis.
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Product Details
Citations (33)
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Mouse ICAM-1/CD54 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54
Gln28-Asn485
Accession # Q3U8M7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)
Immunohistochemistry
5-15 µg/mL
See below
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Mouse ICAM-1 Fc Chimera (Catalog # 796-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry ICAM‑1/CD54 in Mouse Testis. View Larger

ICAM‑1/CD54 in Mouse Testis. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Citations for Mouse ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance and Neurovascular Adhesion Molecule Expression
    Authors: A Niraula, Y Wang, JP Godbout, JF Sheridan
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  2. Complement activation contributes to perioperative neurocognitive disorders in mice
    Authors: C Xiong, J Liu, D Lin, J Zhang, N Terrando, A Wu
    J Neuroinflammation, 2018;15(1):254.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
    Authors: CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao
    Sci Rep, 2017;7(1):717.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
    Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
    J Am Heart Assoc, 2017;6(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  5. Interferon-? treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sj�gren's Syndrome
    Authors: Z Meng, W Klinngam, MC Edman, SF Hamm-Alvar
    PLoS ONE, 2017;12(9):e0184781.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
    Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
    Sci Rep, 2017;7(1):365.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  7. Epidermal RAF prevents allergic skin disease
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  8. Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
    Authors: Goh Q, Dearth C, Corbett J, Pierre P, Chadee D, Pizza F
    Exp Cell Res, 2015;331(2):292-308.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Fresh
  9. Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
    Authors: Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V
    PLoS ONE, 2015;10(3):e0120728.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  10. Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
    Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
    J Immunol, 2015;194(11):5061-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  11. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015;10(3):e0119661.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
    Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P
    PLoS ONE, 2014;9(1):e87098.
    Species: Hamster
    Sample Types: Cell Lysates
    Applications: WB
  13. CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
    Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
    PLoS ONE, 2013;8(12):e84479.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response.
    Authors: Dearth C, Goh Q, Marino J, Cicinelli P, Torres-Palsa M, Pierre P, Worth R, Pizza F
    PLoS ONE, 2013;8(3):e58486.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  15. Peptide inhibitor of NF-kappaB translocation ameliorates experimental atherosclerosis.
    Authors: Mallavia B, Recio C, Oguiza A, Ortiz-Munoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C
    Am J Pathol, 2013;182(5):1910-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fluorescence
  16. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: WB
  17. Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation.
    Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
    Am. J. Pathol., 2011;179(1):125-33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  18. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  19. Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
    Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
    Kidney Int., 2009;75(1):52-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  20. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009;39(1):85-95.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  21. Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency.
    Authors: Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K
    J. Cell. Physiol., 2009;220(2):508-14.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  22. Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
    Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
    Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  23. PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms.
    Authors: Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R
    Brain Res., 2008;1244(0):164-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  24. Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.
    Authors: Casos K, Saiz MP, Ruiz-Sanz JI, Mitjavila MT
    Atherosclerosis, 2008;201(2):306-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  25. Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance.
    Authors: Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW
    Arterioscler. Thromb. Vasc. Biol., 2008;28(11):1982-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  26. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007;100(8):1226-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
    Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
    Circulation, 2007;117(3):421-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  28. Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
    Authors: Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC
    Circ. Res., 2007;101(10):1001-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  29. Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
    Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
    Immunity, 2007;27(2):296-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.
    Authors: Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA
    Br. J. Pharmacol., 2006;149(5):591-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  31. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  32. Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
    Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L962-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  33. Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice.
    Authors: Li Z, Luo T, Ning X, Xiong C, Wu A
    Neurosci Lett, 0;687(0):189-195.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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