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Mouse IFN‑ gamma Antibody

  • Specificity
    Detects mouse IFN-gamma  in direct ELISAs. Does not cross-react with rat, cotton rat, human, rhesus macaque, porcine, feline, or canine IFN-gamma.
  • Source
    Monoclonal Hamster IgG Clone # H22
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IFN-gamma
    Accession # P01580
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Schreiber, R. et al. (1985) J. Immunol. 134:1609; Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN-? Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-? Antibody. Recombinant Mouse IFN-? (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-? (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-? Monoclonal Antibody (Catalog # MAB4851). The ND50 is typically 4-20 ng/mL.
Immunocytochemistry
IFN-? in Mouse Splenocytes. IFN-? was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA (left panel) or unstimulated (right panel) using Hamster Anti-Mouse IFN-? Monoclonal Antibody (Catalog # MAB4851) at 15 µg/mL for 3 hours at room temperature. Cells were stained using an anti-hamster IgG secondary antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38%‑44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  • References:
    1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
    2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
    3. Gray, P.W. and D.V. Goeddel (1983) Proc. Natl. Acad. Sci. 80:5842.
    4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
    5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
    6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
    7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
    8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
    9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
  • Long Name:
    Interferon gamma
  • Entrez Gene IDs:
    15978 (Human); 3458 (Human); 396991 (Human); 281237 (Human); 25712 (Rat); 403801 (Canine); 493965 (Feline);
  • Alternate Names:
    IFNG; IFG; IFI; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 7 of 7
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Species
Applications
Sample Type
  1. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
    Authors: El-Behi M, Ciric B, Dai H
    Nat. Immunol., 2011;12(6):568-75.
  2. Lack of TNFR p55 results in heightened expression of IFN-gamma and IL-17 during the development of reactive arthritis.
    Authors: Elicabe RJ, Cargnelutti E, Serer MI, Stege PW, Valdez SR, Toscano MA, Rabinovich GA, Di Genaro MS
    J. Immunol., 2010;185(7):4485-95.
  3. Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
    Authors: Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z
    J. Immunol., 2008;180(1):113-21.
    Species: Mouse
    Application: In vivo,
  4. Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent.
    Authors: Srivastava K, Zhang T, Yang N, Sampson H, Li XM
    Clin. Exp. Allergy, 2010;40(11):1678-88.
    Species: Mouse
    Application: In vivo,
  5. Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17.
    Authors: Wildbaum G, Zohar Y, Karin N
    Am. J. Pathol., 2010;176(6):2764-75.
    Species: Mouse
    Application: In vivo,
  6. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
    Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
    J. Clin. Invest., 2007;117(12):3868-3878.
    Species: Mouse
    Application: Neutralization,
  7. Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.
    Authors: Ito H, Ando K, Ishikawa T
    Int. Immunol., 2008;20(7):869-79.
    Species: Mouse
    Application: In vivo,

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