Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IGF-I/IGF-1
Gl33-Ala102
Accession # Q8CAR0

Specificity

Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IGF-I/IGF-1 Antibody

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.

Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.
IGF-I/IGF-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

IGF-I/IGF-1 in Mouse Embryo.

IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse IGF-I/IGF-1 by Western Blot

Detection of Mouse IGF-I/IGF-1 by Western Blot

Activation of IGF signaling pathways 36 and 48 h after i.p. injection of paraquat. (A) WB detection of IGF-I from blood and of key signal transduction proteins in IGF pathways (P-tyrosine-activated forms and total protein) from lung tissue. Gel loading was controlled by vinculin. (B) Quantification of WB by chemiluminescence. Phosphotyrosine-IGF-1R (P-IGF-1R) and P-IRS-1 were detected using a phosphotyrosine-specific antibody after IP. Note that increase in IGF-1R abundance over time was very similar whether detected from IP samples or by direct WB. Tests were performed in 11- to 13-week-old mice. N = 3 per group; mean ± SEM, expressed in arbitrary units. *P < 0.05, Mann–Whitney U-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23898955), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IGF-I/IGF-1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)

Neutralization

Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF-I/IGF-1.

Reviewed Applications

Read 2 reviews rated 4 using AF791 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

Q8CAR0

Additional IGF-I/IGF-1 Products

Product Documents for Mouse IGF-I/IGF-1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse IGF-I/IGF-1 Antibody

For research use only

Citations for Mouse IGF-I/IGF-1 Antibody

Customer Reviews for Mouse IGF-I/IGF-1 Antibody (2)

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  • Mouse IGF-I/IGF-1 Antibody
    Name: Rika Ohkubo
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Mouse
    Verified Customer | Posted 04/18/2019
    Could not detect IGF-1 when I used liver samples from mice.
    Mouse IGF-I/IGF-1 Antibody AF791
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23407451
    Species: Mouse
    Verified Customer | Posted 01/09/2015

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