|IGFBP‑2 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Mouse IGFBP‑2 Antibody. Recombinant Mouse IGFBP‑2 (Catalog # 797-B2) inhibits Recombinant Mouse IGF‑II (Catalog # 791-MG) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF‑II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP‑2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB797). The ND50 is typically 5-20 µg/mL.|
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) precursor protein with a 34 aa residue signal peptide and a 271 aa mature protein. Mouse and human IGFBP-2 share approximately 82% aa identity. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.
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