Mouse IGFBP-2 Antibody MAB797: R&D Systems

Mouse IGFBP-2 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IGFBP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, -6, recombinant mouse IGFBP-1, -3, -5, or -6 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 150101
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑2
    Glu35-Gln305
    Accession # CAA57270
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IGFBP‑2 (Catalog # 797-B2) under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize IGFBP‑2 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.75 µg/mL Recombinant Mouse IGFBP‑2 and 30 ng/mL Recombinant Mouse IGF‑II.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IGFBP‑2 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Mouse IGFBP‑2 Antibody. Recombinant Mouse IGFBP‑2 (Catalog # 797-B2) inhibits Recombinant Mouse IGF‑II (Catalog # 791-MG) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF‑II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP‑2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB797). The ND50 is typically 5-20 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) precursor protein with a 34 aa residue signal peptide and a 271 aa mature protein. Mouse and human IGFBP-2 share approximately 82% aa identity. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.

  • References:
    1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
    2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
    3. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
    4. Landwehr, J. et al. (1993) Gene 124:281.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 2
  • Entrez Gene IDs:
    3485 (Human); 16008 (Mouse)
  • Alternate Names:
    BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity.
    Authors: Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ
    Cancer Prev Res (Phila), 2010;3(10):1222-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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