Mouse IL-17/IL-17A PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
IC421C
Detection of IL‑17/IL‑17A in Mouse splenocytes by Flow Cytometry.
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Citations (1)
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Mouse IL-17/IL-17A PerCP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑17/IL‑17A in Mouse splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-mouse CD3 Monoclonal antibody (10 μg/ml; MAB484), Goat anti-mouse CD28 Polyclonal Antibody (5 μg/mL; AF483), TGF-beta (10 ng/mL; 100-B), Recombinant Mouse IL-23 (20 ng/mL; 1887-ML), Recombinant Mouse IL-6 (40 ng/mL; 406-ML), and Recombinant MouseIL-1 beta (10 ng/mL; 401-ML) for 6 days were stained with (A) Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C) or (B) control antibody (IC108C) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Intracellular Staining by Flow Cytometry Detection of IL-17/IL-17A antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17/IL‑17A in EL‑4 Mouse Cell Line by Flow Cytometry. LPS activated EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-17/IL-17A PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-17/IL-17A

Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+ Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citation for Mouse IL-17/IL-17A PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring
    Authors: ST Babu, X Niu, M Raetz, RC Savani, LV Hooper, J Mirpuri
    JCI Insight, 2018-10-04;3(19):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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