Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB448) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R
IL-17 R, also known
as IL-17 RA, is a widely expressed 120 kDa glycosylated type I protein that
plays a central role in inflammatory responses. IL-17 R associates with IL-17
RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 R ligation
promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple
proinflammatory chemokines. Within the ECD, human IL-17 R shares 72% aa
sequence identity with mouse and rat IL-17 R.
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