Mouse IL-17RA/IL-17R Antibody

Catalog #: MAB4481
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4481
MAB4481-SP

Save 15% on Select RUO Reagents. See Details.

Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry.

3 Images

Product Details

Citations (11)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17RA/IL-17R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.

Source

Monoclonal Rat IgG_2A Clone # 657603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R
Ser32-Trp322
Accession # Q60943

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND₅₀) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Neutralization

View Larger

IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481). The ND₅₀ is typically 0.2-0.8 µg/mL.

Flow Cytometry

View Larger

Detection of Mouse IL-17RA/IL-17R by Flow Cytometry IL-17RA is an alternative and better target than IL-25. (A) IL-17 ligand and receptor family. IL-17A, IL-17A/F, IL-17F, IL-17E (IL-25), IL-17B, IL-17C, and IL-17D are either homodimers or heterodimers. (B) Schematic representation of the experimental design for administering anti-IL-17RA antibody (100 ug) twice in combination with aPD-1+aCTLA-4. (Ci) Tumor growth curve of aIL-17RA treated mice compared with those without anti-IL-17RA treatment. (Cii) Average tumor volumes on day 18. (D) Gradings of immune infiltration on day 25. (E) Flow cytometry plot of percent IL-17RA+ in CD4+ T cell and CD8+ T cell from Lpr mice splenocytes (T cells—CD3+ gate). (F) Flow cytometry analysis of CD69 activation marker expression in IL-17RA+ and IL-17RA− CD4+ T cells. Flow cytometry analysis of IL-17RA in central and effector CD4 T cells. (Gi) Exhausted (PD1+ CD69-) CD4+ T cells presented in mice of previous in vivo experiments. (Gii) Sample exhausted cells gating of representative mice from aPD-1/aCTLA-4 and aPD-1/aCTLA-4/aIL-17RA treatment groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38519059), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

Innovative peptide therapeutics targeting IL17RA to regulate inflammatory responses
Authors: Wang, X;Bao, H;Wang, Y;Wang, Y;Guo, C;Wu, Y;Xu, Y;Li, Y;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice
Authors: Li, T;Hu, L;Qin, C;Li, Y;Song, Z;Jiao, Y;Wang, C;Cui, W;Zhang, L;
Brain sciences
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay
Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia
Authors: Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ;
Brain, behavior, and immunity
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Neutralization
CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
Nature Immunology, 2022-10-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
Cell. Signal., 2020-06-15;73(0):109690.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: Neutralization, Western Blot
Colitis-Induced Th17 Cells Increase the Risk for Severe Subsequent Clostridium difficile Infection
Authors: Mahmoud M. Saleh, Alyse L. Frisbee, Jhansi L. Leslie, Erica L. Buonomo, Carrie A. Cowardin, Jennie Z. Ma et al.
Cell Host & Microbe
Interleukin-17A participates in podocyte injury by inducing IL-1? secretion through ROS-NLRP3 inflammasome-caspase-1 pathway
Authors: J Yan, Y Li, H Yang, L Zhang, B Yang, M Wang, Q Li
Scand. J. Immunol., 2018-03-05;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Interleukin-17A Promotes Lung Tumor Progression Through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade
Authors: EA Akbay, S Koyama, Y Liu, R Dries, LE Bufe, M Silkes, MM Alam, DM Magee, R Jones, M Jinushi, M Kulkarni, J Carretero, X Wang, T Warner-Hat, JD Cavanaugh, A Osa, A Kumanogoh, GJ Freeman, MM Awad, DC Christiani, R Bueno, PS Hammerman, G Dranoff, KK Wong
J Thorac Oncol, 2017-05-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents
Authors: AW Qiu, QH Liu, JL Wang
Cell. Physiol. Biochem, 2017-02-21;41(3):960-972.
Species: Mouse, Rat
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
Proc Natl Acad Sci USA, 2016-09-26;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
J Immunol, 2013-03-01;190(7):3427-37.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization