Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.
Monoclonal Rat IgG2A Clone # 657603
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R Ser32-Trp322 Accession # Q60943
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL‑17 (Catalog # 421-ML) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB4481). The ND50 is typically 0.2‑0.8 μg/mL.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R
IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.