Mouse IL-17E/IL-25 Antibody Summary
Accession # NP_542767
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CXCL1/GRO alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Mouse IL‑17E/IL‑25 Antibody. Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL) induces CXCL1/GROa secretion in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa Secretion elicited by Recombinant Mouse IL-17E/IL-25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13992). The ND50is typically 0.005-0.025 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
- Hurst, S.D. et al. (2002) J. Immunol. 169:443.
- Pan, G. et al. (2001) J. Immunol. 167:6569.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse IL-17E/IL-25 Antibody
There are currently no reviews for this product. Be the first to review Mouse IL-17E/IL-25 Antibody and earn rewards!
Have you used Mouse IL-17E/IL-25 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image