CXCL1/GRO alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Mouse IL‑17E/IL‑25 Antibody. |
Recombinant Mouse IL‑17E/IL‑25 (Catalog # 1399-IL) induces CXCL1/GRO alpha secretion in the the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha Secretion elicited by Recombinant Mouse IL‑17E/IL‑25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17E/IL‑25 Monoclonal Antibody (Catalog # MAB13992). The ND50 is typically 0.005-0.025 μg/mL.
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided