CXCL1/GRO alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Mouse IL‑17E/IL‑25 Antibody. |
Recombinant Mouse IL‑17E/IL‑25 (Catalog # 1399-IL) induces CXCL1/GRO alpha secretion in the the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha Secretion elicited by Recombinant Mouse IL‑17E/IL‑25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17E/IL‑25 Monoclonal Antibody (Catalog # MAB13992). The ND50 is typically 0.005-0.025 μg/mL.
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).