Mouse IL-17F Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2057
Product Details
Citations (1)
FAQs
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Mouse IL-17F Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human IL‑17F, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-17F (R&D Systems, Catalog # 2057-IL)
Arg29-Ala153
Accession # NP_665855
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17F (Catalog # 2057-IL)

Mouse IL-17F Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑17F Antibody (Catalog # AF2057)

Standard: Recombinant Mouse IL-17F Protein (Catalog # 2057-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprised of six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17F cDNA encodes a 153 amino acid (aa) protein with a putative 20 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17 sharing approximately 46% aa sequence identity. Mouse and human IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T cells and activated monocytes. Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1, 2, 5). The functions for IL‑17 RC, D, and E are not known. Purified IL-17 R and IL-17B R do not bind IL-17F with high-affinity in vitro. However, binding of IL-17F is detected in cells transfected with IL-17 R, raising the possibility that a co-receptor may be required for IL-17F signaling through IL-17 R (4). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24

Product Datasheets

Citation for Mouse IL-17F Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.
    Authors: Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y
    Immunity, 2009;30(1):108-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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