Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 105828
View all IL-17RA/IL-17R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑17 RA/IL‑17 R
Extracellular domain
Accession # Q60943

Specificity

Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. Shows 50-100% cross‑reactivity with recombinant human IL-17 RA/IL-17 R and no cross‑reactivity with recombinant mouse IL-17B R.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse IL-17RA/IL-17R Antibody

Detection of Mouse IL-17 RA/IL-17 R antibody by Western Blot.

Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB448) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17 RA/IL-17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IL-17RA/IL-17R Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17RA/IL-17R

IL-17 R, also known as IL-17 RA, is a widely expressed 120 kDa glycosylated type I protein that plays a central role in inflammatory responses. IL-17 R associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Q60943

Additional IL-17RA/IL-17R Products

Product Documents for Mouse IL-17RA/IL-17R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-17RA/IL-17R Antibody

For research use only

Related Research Areas

Cytokines and Receptors Research

Citations for Mouse IL-17RA/IL-17R Antibody

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