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Mouse IL-17RA/IL-17R Antibody

Catalog #: MAB4481
11 Citations Datasheet / COA / SDS
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MAB4481
MAB4481-SP

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Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry.
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Citations (11)
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Mouse IL-17RA/IL-17R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.
Source
Monoclonal Rat IgG2A Clone # 657603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R
Ser32-Trp322
Accession # Q60943
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-17 RA/IL-17 R antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Neutralization IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. View Larger

IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481). The ND50 is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.

Long Name
Interleukin 17 Receptor
Entrez Gene IDs
23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)
Alternate Names
CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

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Citations for Mouse IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Innovative peptide therapeutics targeting IL17RA to regulate inflammatory responses
    Authors: Wang, X;Bao, H;Wang, Y;Wang, Y;Guo, C;Wu, Y;Xu, Y;Li, Y;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice
    Authors: Li, T;Hu, L;Qin, C;Li, Y;Song, Z;Jiao, Y;Wang, C;Cui, W;Zhang, L;
    Brain sciences
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  3. Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia
    Authors: Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Immunohistochemistry, Neutralization
  4. CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
    Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
    Nature Immunology, 2022-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
    Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
    Cell. Signal., 2020-06-15;73(0):109690.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Neutralization, Western Blot
  6. Colitis-Induced Th17 Cells Increase the Risk for Severe Subsequent Clostridium difficile Infection
    Authors: Mahmoud M. Saleh, Alyse L. Frisbee, Jhansi L. Leslie, Erica L. Buonomo, Carrie A. Cowardin, Jennie Z. Ma et al.
    Cell Host & Microbe
  7. Interleukin-17A participates in podocyte injury by inducing IL-1? secretion through ROS-NLRP3 inflammasome-caspase-1 pathway
    Authors: J Yan, Y Li, H Yang, L Zhang, B Yang, M Wang, Q Li
    Scand. J. Immunol., 2018-03-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Interleukin-17A Promotes Lung Tumor Progression Through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade
    Authors: EA Akbay, S Koyama, Y Liu, R Dries, LE Bufe, M Silkes, MM Alam, DM Magee, R Jones, M Jinushi, M Kulkarni, J Carretero, X Wang, T Warner-Hat, JD Cavanaugh, A Osa, A Kumanogoh, GJ Freeman, MM Awad, DC Christiani, R Bueno, PS Hammerman, G Dranoff, KK Wong
    J Thorac Oncol, 2017-05-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents
    Authors: AW Qiu, QH Liu, JL Wang
    Cell. Physiol. Biochem, 2017-02-21;41(3):960-972.
    Species: Mouse, Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  10. Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
    Proc Natl Acad Sci USA, 2016-09-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
    Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
    J Immunol, 2013-03-01;190(7):3427-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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