Mouse IL-18 R alpha /IL-1 R5 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
  • Source
    Monoclonal Rat IgG2A Clone # 112624
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha
    Ser19-Gly326
    Accession # Q61098
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IL‑18 R alpha /IL‑1 R5
  • Neutralization
    Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutraliza­tion by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.
  • Long Name:
    Interleukin 18 Receptor alpha
  • Entrez Gene IDs:
    8809 (Human); 16182 (Mouse)
  • Alternate Names:
    CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
    Authors: Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G
    Nat Med, 2015;21(7):820-6.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  2. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
    Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
    J. Immunol., 2011;187(3):1333-46.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
    Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
    J. Immunol., 2007;178(3):1948-59.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Protection of CD8+ T cells from activation-induced cell death by IL-18.
    Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
    J. Leukoc. Biol., 2007;82(1):142-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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