Mouse IL-18 R alpha /IL-1 R5 Antibody

Catalog # Availability Size / Price Qty
MAB12161-SP
MAB12161-100
MAB12161-500
IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha /IL‑1 R5 Antibody.
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Citations (4)
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Mouse IL-18 R alpha /IL-1 R5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
Source
Monoclonal Rat IgG2A Clone # 112624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha
Ser19-Gly326
Accession # Q61098
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑18 R alpha /IL‑1 R5
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha /IL‑1 R5 Antibody. View Larger

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Citations for Mouse IL-18 R alpha /IL-1 R5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
    Authors: Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G
    Nat Med, 2015-06-22;21(7):820-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
    Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
    J. Immunol., 2011-06-29;187(3):1333-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Protection of CD8+ T cells from activation-induced cell death by IL-18.
    Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
    J. Leukoc. Biol., 2007-03-30;82(1):142-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
    Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
    J. Immunol., 2007-02-01;178(3):1948-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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