Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
Monoclonal Rat IgG2A Clone # 112624
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha Ser19-Gly326 Accession # Q61098
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse IL‑18 R alpha /IL‑1 R5
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.
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