Mouse IL-1ra/IL-1F3 Antibody

Catalog # Availability Size / Price Qty
MAB4802-100
MAB4802-SP
IL‑1ra/IL‑1F3  in A431 Human Cell Line.
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Mouse IL-1ra/IL-1F3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑1ra/IL‑1F3 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 962802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL‑1F3
Arg27-Gln178
Accession # P258085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑1ra/IL‑1F3  in A431 Human Cell Line. View Larger

IL‑1ra/IL‑1F3 in A431 Human Cell Line. IL‑1ra/IL‑1F3 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rat Anti-Mouse IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB4802) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25 kDa. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name
Interleukin 1 Receptor Antagonist
Entrez Gene IDs
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names
DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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