Detects mouse IL‑1ra/IL-1F3 in ELISAs and Western blots. In sandwich immunoassays, approximately 45% cross-reactivity with recombinant rat IL‑1ra is observed and less than 1% cross-reactivity with recombinant human IL-1ra, recombinant porcine IL-1ra, and recombinant equine IL‑1ra is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Mouse IL-1ra/IL-1F3 Protein (Catalog # 480-RM)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
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