Mouse IL-1ra/IL-1F3 Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-1ra/IL-1F3 in ELISAs and Western blots. In sandwich ELISAs, approximately 45% cross-reactivity with recombinant rat IL‑1ra/IL‑1F3 and less than 1% cross-reactivity with recombinant human IL‑1ra/IL‑1F3, recombinant porcine IL‑1ra/IL‑1F3, and recombinant equine IL‑1ra/IL‑1F3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL‑1ra/IL-1F3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑1ra/IL‑1F3 (Catalog # 480-RM)
    • Mouse IL-1ra/IL-1F3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse IL‑1ra/IL‑1F3 Antibody (Catalog # AF-480-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑1ra/IL‑1F3 Biotinylated Antibody (Catalog # BAF480)
  • ELISA Standard
     
    Recombinant Mouse IL-1ra/IL-1F3 Protein (Catalog # 480-RM)
  • Neutralization
    Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line [Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272]. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 150 ng/mL Recombinant Mouse IL‑1ra/IL‑1F3, 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Mouse IL‑1ra/IL‑1F3 Antibody. Recombinant Mouse IL‑1ra/IL‑1F3 (Catalog # 480‑RM) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200‑LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL‑1ra/IL‑1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

  • Long Name:
    Interleukin 1 Receptor Antagonist
  • Entrez Gene IDs:
    3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
  • Alternate Names:
    DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Effective combinatorial immunotherapy for castration-resistant prostate cancer
    Authors: X Lu, JW Horner, E Paul, X Shang, P Troncoso, P Deng, S Jiang, Q Chang, DJ Spring, P Sharma, JA Zebala, DY Maeda, YA Wang, RA DePinho
    Nature, 2017;543(7647):728-732.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague.
    PLoS Pathog, 2015;11(5):e1004921.
    Species: Mouse
    Sample Type: In Vivo
  3. Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats.
    Authors: Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y
    PLoS ONE, 2014;9(3):e90950.
    Species: Rat
    Sample Type: In Vivo
    Application: Neut
  4. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
    Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
    Crit. Care Med., 2009;37(5):1567-73.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  5. Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury.
    Authors: Ortiz LA, Dutreil M, Fattman C, Pandey AC, Torres G, Go K, Phinney DG
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(26):11002-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  7. Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
    Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
    Neuroscience, 2005;132(3):625-31.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  8. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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