IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL‑1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑1 Rrp2/IL‑1 R6
Asp22-Arg338 (Ala158Val)
Accession # Q9ERS7
Asp22-Arg338 (Ala158Val)
Accession # Q9ERS7
Specificity
Detects mouse IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs, less than 60% with recombinant rat IL-1 Rrp2 and recombinant human IL-1 Rrp2 is observed and less than 5% cross‑reactivity with recombinant mouse (rm) IL-1 R4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse IL-1Rrp2/IL-1R6 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL‑1 Rrp2/IL‑1 R6 (Catalog # 2354-RP)
Sample: Recombinant Mouse IL‑1 Rrp2/IL‑1 R6 (Catalog # 2354-RP)
Reviewed Applications
Read 1 review rated 5 using AF2354 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-1Rrp2/IL-1R6
References
- Dunne, A. and L.A. O’Neill, (2003) Sci. STKE Feb 25 (171):re3.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Lovenberg, T.S. et al. (1996) J. Neuroimmunol. 70:113.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 1 Receptor-like 2
Alternate Names
IL-1 R6, IL-1 Rrp2, IL-1RL2, IL-1Rrp2, IL1RL2, IL1Rrp2
Gene Symbol
IL1RL2
UniProt
Additional IL-1Rrp2/IL-1R6 Products
Product Documents for Mouse IL-1Rrp2/IL-1R6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-1Rrp2/IL-1R6 Antibody
For research use only
Related Research Areas
Citations for Mouse IL-1Rrp2/IL-1R6 Antibody
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Application: Flow CytometrySample Tested: 293T human embryonic kidney cell lineSpecies: MouseVerified Customer | Posted 12/13/2019
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
