Mouse IL-21R Antibody

Name: Mouse IL-21R Antibody
Brand: R&D Systems
SKU: MAB596
Price: 539 USD
Availability: InStock

R&D Systems | Catalog # MAB596

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Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 155502

View all IL-21R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-21 R
Leu21-Pro236
Accession # Q9JHX3

Specificity

Detects mouse IL-21 R in direct ELISAs and Western blots. Does not cross-react with recombinant human IL-21 R.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Applications for Mouse IL-21R Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑21 R Subunit Fc Chimera (Catalog # 596-MR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

The interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor) is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 R is a 529 amino acid (aa) residue protein with a 19 aa signal peptide, a 217 aa extracellular domain, an 18 aa transmembrane domain, and a 275 aa cytoplasmic domain. Mouse and human IL-21 R share 62% aa identity. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B and natural killer lineage. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and natural killer cell maturation.