Mouse IL-23 R Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects endogenous mouse IL-23 R by flow cytometry.
  • Source
    Monoclonal Rat IgG1 Clone # 753317
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
    Accession # Q8K4B4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3
(Catalog # MAB484),5 μg/mL Anti-CD28 (Catalog # AF483),
10 ng/mL Recombinant Mouse TGF- beta 1 (Catalog # 100-B),
20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML),40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML),and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML)for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4APC‑conjugated Monoclonal Antibody (Catalog # FAB544A)and either (A) Rat Anti-Mouse IL‑23 R PE‑conjugated Monoclonal Antibody(Catalog # FAB16861P) or (B) Rat IgG1 PhycoerythrinIsotype Control (Catalog # IC005P).View our protocol for StainingMembrane-associated Proteins.
Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL‑23 R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB16861G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23 R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

  • References:
    1. Oppmann, B. et al. (2000) Immunity 13:715.
    2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
    3. Parham, C. et al. (2002) J. Immunol. 168:5448.
    4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
    5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  • Long Name:
    Interleukin 23 Receptor
  • Entrez Gene IDs:
    149233 (Human); 209590 (Mouse)
  • Alternate Names:
    IBD17; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC005G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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