Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3 |
(Catalog # MAB484),5 μg/mL Anti-CD28 (Catalog # AF483),
10 ng/mL Recombinant Mouse TGF- beta 1 (Catalog # 100-B),
20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML),40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML),and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML)for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4APC‑conjugated Monoclonal Antibody (Catalog # FAB544A)and either (A) Rat Anti-Mouse IL‑23 R PE‑conjugated Monoclonal Antibody(Catalog # FAB16861P) or (B) Rat IgG1 PhycoerythrinIsotype Control (Catalog # IC005P).View our protocol for StainingMembrane-associated Proteins.
|Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL‑23 R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB16861G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.