IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.
Mouse IL-4 Quantikine ELISA Kit
R&D Systems | Catalog # M4000B
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
2 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
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Product Summary for Mouse IL-4 Quantikine ELISA Kit
The Quantikine Mouse IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-4 accurately. Results obtained using natural mouse IL-4 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-4.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 20.1 | 58.6 | 199 | 20.7 | 58.8 | 203 |
| Standard Deviation | 1.3 | 1.8 | 8.9 | 1.2 | 2.7 | 10.6 |
| CV% | 6.5 | 3.1 | 4.5 | 5.8 | 4.6 | 5.2 |
Recovery for Mouse IL-4 Quantikine ELISA Kit
The recovery of mouse IL-4 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=8) | 95 | 84-109 |
| Serum (n=7) | 95 | 88-118 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-4 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-4 Quantikine ELISA Kit
Mouse IL-4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-4
Long Name
Interleukin 4
Alternate Names
BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12
Entrez Gene IDs
Gene Symbol
IL4
Additional IL-4 Products
Product Documents for Mouse IL-4 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-4 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse IL-4 Quantikine ELISA Kit
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Customer Reviews for Mouse IL-4 Quantikine ELISA Kit (6)
4.8 out of 5
6 Customer Ratings
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Sample Tested: Cell culture supernatantVerified Customer | Posted 11/28/2022Cell culture supernatant diluted 1:100
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Sample Tested: EDTA Plasma and Tissue HomogenatesVerified Customer | Posted 07/10/2020
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 08/06/2018
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Sample Tested: SerumVerified Customer | Posted 03/30/2018
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Sample Tested: Mouse lung lysatesVerified Customer | Posted 07/06/2017This ELISA works awesomely in mouse lung samples.
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Sample Tested: Lung tissue and RAW 264.7 mouse monocyte/macrophage cell lineVerified Customer | Posted 05/02/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-4 Signaling Pathways
IL-4 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
