Mouse IL-4R alpha Antibody

Catalog # Availability Size / Price Qty
MAB530
MAB530-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse IL-4R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-4 R alpha in direct ELISAs and Western blots. Shows less than 1% cross‑reactivity with recombinant human IL-4 R alpha.
Source
Monoclonal Rat IgG2B Clone # 129801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-4 R alpha
Ile26-Arg233
Accession # P16382
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑4 R alpha Fc Chimera (Catalog # 530-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component ( beta chain) of the IL-4 receptor complex.

cDNA clones for the ligand binding chain (IL-4 R alpha ) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4 R alpha is an approximately 140 kDa transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing and that encodes a soluble secreted form of IL-4 R alpha has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R alpha has also been identified in mouse biological fluids and murine cell culture supernatants.

Native or recombinant murine soluble IL-4 R alpha, as well as recombinant human soluble IL-4 R alpha, can bind IL-4 with the same affinity as the membrane bound IL-4 R alpha. Soluble IL-4 R alpha is a competitive inhibitor of IL-4 and has been shown to neutralize effectively many IL-4-mediated responses both in vivo and in vitro.

Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Product Datasheets

Citations for Mouse IL-4R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Multivalent Targeting Based Delivery of Therapeutic Peptide using AP1-ELP Carrier for Effective Cancer Therapy
    Theranostics, 2016;6(12):2235-2249.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. IL-4, a direct target of miR-340/429, is involved in radiation-induced aggressive tumor behavior in human carcinoma cells
    Authors: ES Kim, YE Choi, SJ Hwang, YH Han, MJ Park, IH Bae
    Oncotarget, 2016;7(52):86836-86856.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. IL-21 administration into the nostril alleviates murine allergic rhinitis.
    Authors: Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O
    J. Immunol., 2007;179(10):7157-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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