Mouse IL-6R alpha Antibody (255820)

R&D Systems | Catalog # MAB18301

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 255820
View all IL-6R alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha
Leu20-Glu357
Accession # P22272

Specificity

Detects mouse IL-6R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-6 R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IL-6R alpha Antibody (255820)

IL-6R alpha antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

IL-6R alpha in Mouse Splenocytes.

IL-6R alpha was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-6R alpha Monoclonal Antibody (Catalog # MAB18301) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse IL-6R alpha Antibody (255820)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL-6R alpha (Catalog # 1830-SR)

Reviewed Applications

Read 4 reviews rated 4.5 using MAB18301 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds  IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

References

  1. Yamasaki, K. et al. (1988) Science 241:825.
  2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
  3. Hibi, M. et al. (1990) Cell 63:1149.
  4. Saito, M. et al. (1992) J. Immunol. 148:4066.
  5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
  6. Jones, S.A. et al. (2001) FASEB J. 15:43.
  7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Long Name

Interleukin 6 Receptor alpha

Alternate Names

CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Gene Symbol

IL6R

UniProt

P22272

Additional IL-6R alpha Products

Product Documents for Mouse IL-6R alpha Antibody (255820)

Certificate of Analysis

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Product Specific Notices for Mouse IL-6R alpha Antibody (255820)

For research use only

Citations for Mouse IL-6R alpha Antibody (255820)

Customer Reviews for Mouse IL-6R alpha Antibody (255820) (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse IL-6R alpha Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cancerous basophil cells
    Species: Mouse
    Verified Customer | Posted 12/14/2021
    Mouse IL-6R alpha Antibody (255820) MAB18301
  • Mouse IL-6R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 05/20/2019
  • Mouse IL-6 R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 07/15/2018
    Mouse IL-6R alpha Antibody (255820) MAB18301
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 22295972
    Species: Mouse
    Verified Customer | Posted 02/10/2015

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Protocols

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