Detects mouse IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-9.
Monoclonal Rat IgG2B Clone # 222622
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9 Gln19-Pro144 Accession # P15247.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Moreau, F. J. et al. (1988) Nature 336:690. The Neutralization Dose (ND50) is typically 0.5-2.0 ng/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL‑9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑9 Monoclonal Antibody (Catalog # MAB4091). The ND50 is typically 0.5-2.0 ng/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-9 was originally identified as a T cell-derived T cell growth factor
III/P40 which could support the long term growth of certain mouse T
helper clones in the absence of antigen or antigen-presenting cells.
IL-9 can also prolong the in vitro survival of other T cell
clones as well as potentiate the IL-2 dependent proliferation of mouse
fetal thymocytes. However, this cytokine has no growth-stimulating
activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9
also has mast cell enhancing activity (MEA) and can enhance the mIL-3-
or mIL-4-dependent proliferation of mouse bone marrow-derived mast
cells. Furthermore, IL-9 will synergize with erythropoietin to support
erythroid colony formation in vitro. The mouse IL-9 cDNA
encodes a 144 amino acid residue precursor protein with an 18 amino acid
signal peptide that is cleaved to form the mature cysteine-rich protein
with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four
potential N-linked glycosylation sites and the native mIL-9 is a highly
glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence
homology. Although mouse IL-9 is active on human cells, human IL-9 is
not active on mouse cells.
Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.
Entrez Gene IDs:
3578 (Human); 16198 (Mouse)
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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