Mouse IL-9 Antibody

Catalog # Availability Size / Price Qty
MAB4091-SP
MAB4091-100
MAB4091-500
Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody.
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Product Details
Citations (4)
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Mouse IL-9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-9.
Source
Monoclonal Rat IgG2B Clone # 222622
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9
Gln19-Pro144
Accession # P15247.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Moreau, F. J. et al. (1988) Nature 336:690. The Neutralization Dose (ND50) is typically 0.5-2.0 ng/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. View Larger

Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL-9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-9 Monoclonal Antibody (Catalog # MAB4091). The ND50 is typically 0.5-2.0 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence homology. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.

References
  1. Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.
Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Product Datasheets

Citations for Mouse IL-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Nitric oxide enhances Th9 cell differentiation and airway inflammation.
    Authors: Niedbala W, Besnard A, Nascimento D, Donate P, Sonego F, Yip E, Guabiraba R, Chang H, Fukada S, Salmond R, Schmitt E, Bopp T, Ryffel B, Liew F
    Nat Commun, 2014;5(0):4575.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.
    Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH
    Nat. Immunol., 2010;11(6):527-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. IL-9 induces differentiation of TH17 cells and enhances function of FoxP3+ natural regulatory T cells.
    Authors: Elyaman W, Bradshaw E, Uyttenhove C, Dardalhon V, Awasthi A, Imitola J, Bettelli E, Oukka M, Van Snick J, Renauld J, Kuchroo V, Khoury S
    Proc Natl Acad Sci U S A, 0;106(31):12885-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. TH9 cells are required for tissue mast cell accumulation during allergic inflammation.
    Authors: Sehra S, Yao W, Nguyen E, Glosson-Byers N, Akhtar N, Zhou B, Kaplan M
    J Allergy Clin Immunol, 0;136(2):433-40.e1.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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