Mouse Intelectin-1/Omentin Antibody Summary
Accession # O88310
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Intelectin‑1/Omentin by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Intelectin (endothelial lectin; also ITLN-1 and omentin) is a 34 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed in intestinal and airway epithelium and appears to participate in insulin signaling and microbe recognition. Intelectin-1 is upregulated in asthma and contributes to allergic airway inflammation. The mouse Intelectin-1 preproprecursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature segment (aa 20-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. Soluble Intelectin-1 can be released from omental adipose tissue and malignant pleural mesothelioma. One fibrinogen-related domain exists in the mature molecule (aa 32-251). Human Intelectin-1 exists as a disulfide-linked glycosylated homotrimer; mouse is unglycosylated and a monomer. Mature mouse Intelectin-1 is 82% aa identical to human Intelectin-1.
Citation for Mouse Intelectin-1/Omentin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
Authors: SS Rao, Y Hu, PL Xie, J Cao, ZX Wang, JH Liu, H Yin, J Huang, YJ Tan, J Luo, MJ Luo, SY Tang, TH Chen, LQ Yuan, EY Liao, R Xu, ZZ Liu, CY Chen, H Xie
Bone Res, 2018;6(0):9.
Sample Types: Tissue Homogenates
Applications: Western Blot
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