Mouse Intelectin-1/Omentin Antibody

Catalog # Availability Size / Price Qty
MAB8074
MAB8074-SP
Mouse Intelectin-1/Omentin Antibody in Western Blot
2 Images
Product Details
Citations (1)
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Mouse Intelectin-1/Omentin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Intelectin‑1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 746420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Intelectin‑1/Omentin
Ala20-Ser298
Accession # O88310
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Intelectin‑1/Omentin by Western Blot. View Larger

Detection of Mouse Intelectin‑1/Omentin by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Intelectin‑1/Omentin in Mouse Intestine. View Larger

Intelectin‑1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/Omentin

Intelectin (endothelial lectin; also ITLN-1 and omentin) is a 34 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed in intestinal and airway epithelium and appears to participate in insulin signaling and microbe recognition. Intelectin-1 is upregulated in asthma and contributes to allergic airway inflammation. The mouse Intelectin-1 preproprecursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature segment (aa 20-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. Soluble Intelectin-1 can be released from omental adipose tissue and malignant pleural mesothelioma. One fibrinogen-related domain exists in the mature molecule (aa 32-251). Human Intelectin-1 exists as a disulfide-linked glycosylated homotrimer; mouse is unglycosylated and a monomer. Mature mouse Intelectin-1 is 82% aa identical to human Intelectin-1.

Entrez Gene IDs
55600 (Human); 16429 (Mouse)
Alternate Names
Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1

Product Datasheets

Citation for Mouse Intelectin-1/Omentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
    Authors: SS Rao, Y Hu, PL Xie, J Cao, ZX Wang, JH Liu, H Yin, J Huang, YJ Tan, J Luo, MJ Luo, SY Tang, TH Chen, LQ Yuan, EY Liao, R Xu, ZZ Liu, CY Chen, H Xie
    Bone Res, 2018;6(0):9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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