Mouse ISLR-2 Antibody

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Detection of Mouse ISLR‑2 by Western Blot.
2 Images
Product Details
Citations (2)
Supplemental Products

Mouse ISLR-2 Antibody Summary

Species Reactivity
Detects mouse ISLR-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human ISLR-2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse ISLR-2
Accession # Q5RKR3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
5-15 µg/mL
Immersion fixed mouse embryonic retina cells (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ISLR‑2 antibody by Western Blot. View Larger

Detection of Mouse ISLR‑2 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ISLR-2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry ISLR-2 antibody in Embryonic Mouse Brainstem by Immunohistochemistry (IHC-Fr). View Larger

ISLR‑2 in Embryonic Mouse Brainstem. ISLR-2 was detected in immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.) using Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ISLR-2

ISLR2 (Ig‑superfamily containing LRR protein 2; also SST273) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mouse ISLR2 is 745 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 132 aa cytoplasmic region. The ECD shows multiple LRRs (aa 21‑231) and one Ig‑like domain (aa 251‑374). Mouse ISLR2 apparently has one alternate start site at Met130. Over aa 20‑590, mouse ISLR2 is 89% and 98% aa identical to human and rat ISLR2, respectively.

Long Name
Immunoglobulin Superfamily Containing Leucine-rich Repeat 2
Entrez Gene IDs
57611 (Human); 320563 (Mouse)
Alternate Names
immunoglobulin superfamily containing leucine-rich repeat 2; ISLR2; ISLR-2; KIAA1465; leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein; LINX; UNQ1885/PRO4329

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Citations for Mouse ISLR-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
    Authors: S Abudureyim, N Asai, A Enomoto, L Weng, H Kobayashi, X Wang, C Chen, S Mii, M Takahashi
    Sci Rep, 2018-05-08;8(1):7292.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot


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